Structural characterization of a ribose-5-phosphate isomerase B from the pathogenic fungus Coccidioides immitis
© Edwards et al; licensee BioMed Central Ltd. 2011
Received: 1 July 2011
Accepted: 13 October 2011
Published: 13 October 2011
Ribose-5-phosphate isomerase is an enzyme that catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate. This family of enzymes naturally occurs in two distinct classes, RpiA and RpiB, which play an important role in the pentose phosphate pathway and nucleotide and co-factor biogenesis.
Although RpiB occurs predominantly in bacteria, here we report crystal structures of a putative RpiB from the pathogenic fungus Coccidioides immitis. A 1.9 Å resolution apo structure was solved by combined molecular replacement and single wavelength anomalous dispersion (SAD) phasing using a crystal soaked briefly in a solution containing a high concentration of iodide ions. RpiB from C. immitis contains modest sequence and high structural homology to other known RpiB structures. A 1.8 Å resolution phosphate-bound structure demonstrates phosphate recognition and charge stabilization by a single positively charged residue whereas other members of this family use up to five positively charged residues to contact the phosphate of ribose-5-phosphate. A 1.7 Å resolution structure was obtained in which the catalytic base of C. immitis RpiB, Cys76, appears to form a weakly covalent bond with the central carbon of malonic acid with a bond distance of 2.2 Å. This interaction may mimic that formed by the suicide inhibitor iodoacetic acid with RpiB.
The C. immitis RpiB contains the same fold and similar features as other members of this class of enzymes such as a highly reactive active site cysteine residue, but utilizes a divergent phosphate recognition strategy and may recognize a different substrate altogether.
Ribose-5-phosphate isomerases catalyze the interconversion of ribulose-5-phosphate and ribose-5-phosphate as an important part of the pentose phosphate pathway . Ribose-5-phosphate is used in nucleotide and co-factor biosynthesis. As with other isomerases, ribose-5-phosphate catalyzes this reaction at near equilibrium. Two types of ribose-5-phosphate isomerases exist, RpiA and RpiB, which share little structural homology and have distinct active sites and mechanisms of action . Most organisms such as Escherichia coli contain both RpiA and RpiB, but other organisms contain only one class of Rpi. In E. coli, a double rpia/rpib knockout exhibited severely impaired growth . RpiB occurs almost exclusively in bacteria.
RpiB is a member of the LacAB_rpiB superfamily of proteins (PFAM PF02502). RpiB from different organisms may have different substrate specificities and several annotated RpiB enzymes act upon allose-5-phosphate rather than or in addition to ribose-5-phosphate and are thus named AlsI. Indeed, one of the first crystal structures solved for a member of this family was the E. coli RpiB/AlsI . Given the important cellular role of ribose-5-phosphate isomerases and that mammals only have RpiA, interest has been generated in RpiB enzymes as potential drug targets, especially in pathogenic organisms, many of which only have RpiB. E. coli RpiB inhibitors have been described  and inhibitor-bound crystal structures have been determined for RpiB from Mycobacterium tuberculosis which causes tuberculosis and Trypanosma cruzi, the causative agent of trypanosomiasis.
Coccidioides immitis is a pathogenic fungus that causes coccidioidomycosis, also known as Valley Fever [7–9]. One gene (CIMG_07932) encodes a putative uncharacterized protein that contains high sequence homology with RpiB enzymes from closely related organisms. Specifically, the CIMG_07932 gene product contains 98% sequence identity to an Rpi from C. posadasii, 89% sequence identity to an Rpi from the non-pathogenic fungus Uncinocarpus reesii, and 78% sequence identity to an RpiB from Paracoccidioides brasiliensis. We have undertaken structural analysis of RpiB from C. immitis and present here crystal structures in apo and ligand bound forms.
Results and Discussion
Structure determination of C. immitis RpiB
Data collection, phasing and refinement statistics
a = 103.2 Å, b = 49.9 Å, c = 62.0 Å, β = 108.6°
a = 77.7 Å, b = 85.2 Å, c = 96.3 Å
a = 77.5 Å, b = 84.4 Å, c = 96.2 Å
Resolution range (Å)
FOM (Phaser EP)
RMSD bonds (Å)
RMSD angles (°)
Mean B-factor (Å2)
Molprobity score 
1.42 (97th percentile)
Interestingly, the active site contains electron density consistent with an oxidized cysteine residue (Cys76) in both protomers (Figure 1). Although the exact oxidation state of the cysteine residue was not determined, the electron density maps were most consistent with two conformations of the sulfenic state rather than sulfinic or sulfonic acid. The oxygen on Cys76 appears to adopt two different orientations contacting the backbone nitrogen and side chain hydroxyl of Thr78 in one orientation and the backbone nitrogens of Gly81 and Val82 in the other. The oxidized nature of Cys76 may imply that this residue, thought to be the catalytic base, is highly reactive. The high concentration solution of sodium iodide used for phasing likely contains iodine, indicated by a light yellow color. Iodine is the most probable source for the oxidation of Cys76, which was not oxidized in the other two C. immitis RpiB structures (see below). Oxidation is unlikely to have occurred as a result of radiation damage since the data were collected in house under cryogenic temperatures. Oxidized cysteines have been observed previously in the presence of iodide ions [17, 18] and also for other RpiB enzymes (PDB entries 1O1X  and 3C5Y, no primary citation) determined in the absence of iodide.
Comparison with other ribose-5-phosphate isomerases
Comparison of the global structure of C. immitis RpiB with RpiB enzymes from other organisms
Similar Cα atoms
The C. immitis RpiB contains the canonical open (α,β) Rossman fold, a common fold for proteins that bind nucleotides or nucleotide-like molecules . As determined by the Protein Interfaces, Surfaces and Assemblies (PISA) server , the quaternary structure appears to be dimeric (Figure 1) with 7408 Å2 of buried surface (12,690 Å2 of surface area for the dimer). However, based on the structure, PISA also predicts a tetrameric structure (generated by crystal symmetry) in which 17,610 Å2 of surface area is buried (22,730 Å2 of surface area for the tetramer). The tetrameric structure of C. immitis RpiB is most likely a dimer of dimers with one strong dimer and the secondary weaker dimer interaction that makes the tetramer. This quaternary structure is consistent with the two ligand bound C. immitis RpiB described below in a different crystal form, and with previous X-ray structural characterization of RpiB, such as that from E. coli. Dynamic light scattering (DLS) experiments indicated a monodisperse protein with an approximate molecular weight of 80 kDa which is consistent with a tetramer in solution. DLS measurement on E. coli RpiB also indicated a tetramer in solution . Thermofluor analysis indicated a stable protein with a melting temperature of 52°C. The melting temperature was unaffected by the presence of ribose-5-phosphate, ribose-5-phosphate with MnCl2, or iodoacetate.
C. immitis RpiB substrate recognition
Given the substantial differences in the active site of the C. immitis RpiB in comparison with other structurally and functionally characterized RpiB enzymes, it is possible that the C. immitis enzyme utilizes a substrate different than ribose-5-phosphate/ribulose-5-phosphate. In addition to the single positively charged residue that interacts with the phosphate, the highly conserved histidine involved in ring opening in the catalytic mechanism (His102" in C. thermocellum) has been replaced with Ser109" in the C. immitis structure (Figure 4). Given the position of the phosphate in the C. immitis structure, we suspect that this enzyme may isomerize shorter chain substrates, although confirmation of substrate activity will require further structural and biochemical characterization. Furthermore, C. immitis contains another gene (CIMG_09662) that encodes a putative uncharacterized protein which contains 99% sequence identity to RpiA from C. posadasii (gene CPC735_023760). Therefore, this essential function of the pentose phosphate pathway may be accomplished by RpiA, allowing RpiB to have evolved with an altered substrate specificity.
C. immitis RpiB covalent inhibition
The chemical reaction of Cys76 with iodoacetate is expected to form a Cys-CH2COOH adduct. The conformation of either of the carboxylates of malonic acid may be reflective of the carboxylate of the covalent adduct formed with iodoacetate. One of the carboxylates of malonic acid forms hydrogen bonds with the backbone amides of Thr78 (conserved as a serine or threonine, 3.0 Å) and the universally conserved Gly77 (2.9 Å) as well as the side chain of the universally conserved Asp16 (2.6 Å). The hydrogen bond with Asp16 is unlikely to occur at neutral pH where both carboxylates are expected to be deprotonated and negatively charged. In contrast, the interaction is well ordered in the pH 5.0 crystal. Given an approximate pKa of ~4.8, at pH 5.0 about half of the carboxylates should be protonated. The second malonic acid carboxylate forms hydrogen bonding interactions with the backbone amide nitrogen of the universally conserved Gly81 (2.5 Å) and with the side chain of the universally conserved Asn110' of the other protomer of the obligate dimeric enzyme (3.0 Å). The interactions of this carboxylate with RpiB may reflect the interactions formed by the iodoacetate covalent adduct. There is a chloride ion in the active site 3.7 Å away from the malonic acid and near Arg105' and Arg120, although this anion is ill-suited to mimic the iodide ion leaving group of iodoacetate because it is on the opposite side of the caboxylate relative to Cys76. An iodide ion resides in the same place as this chloride ion in both protomers of the asymmetric unit in the iodide-phased structure.
Here we present crystal structures of RpiB from the pathogenic fungus C. immitis, which together with the structures from T. cruzi and G. lamblia (Edwards, T.E. et al., unpublished) are the only eukaryotic RpiB crystal structures currently available. These structures reveal the presence of a highly reactive cysteine residue in the active site, thought to be the catalytic base in the isomerase reaction and provide insight into a possible structural mechanism for the inhibition of RpiB by iodoacetate. Furthermore, these structures reveal the basis for phosphate recognition by a single positively charged residue and may indicate that this C. immitis has altered substrate specificity.
Cloning, expression, and purification
The 163-residue Coccidioides immitis putative uncharacterized protein (C. immitis RpiB, UniProt ID: P0CL19, formerly Q1DP31; Gene CIMG_07932, targetDB ID: CoimA.00584.a, E. C. 126.96.36.199) was amplified from genomic DNA and cloned into an expression vector (pAVA0421) encoding an N-terminal histidine affinity tag followed by the human rhinovirus 3C protease cleavage sequence using ligation independent cloning . The full length expressed protein contains the tag sequence MAHHHHHHMGTLEAQTQGPGS followed by the 163-residue C. immitis RpiB. The plasmid is available through the BEI repository (NR-28451). The plasmid was transformed into E. coli BL21 (DE3) R3 Rosetta cells. Starter cultures of LB broth with appropriate antibiotics were grown for ~18 hours at 37°C. Protein was expressed in a LEX bioreactor in ZYP-5052 auto-induction media  in the presence of antibiotics in 2 liters of sterilized auto-induction media inoculated with the overnight starter culture. After 24 hours at 25°C the temperature was reduced to 15°C for an additional 60 hours. The sample was centrifuged at 4000 × g for 20 minutes at 4°C. Cell paste was flash frozen in liquid nitrogen and stored at -80°C. The frozen cells were re-suspended in 20 mM HEPES pH 7.0, 300 mM NaCl, 5% w/v glycerol, 0.5% w/v CHAPS, 30 mM imidazole, 10 mM MgCl2, 1 mM TCEP, 250 μg/ml AEBSF, 200 μl of lysozyme (100 mg/mL), 5 tablets of Roche protease inhibitor tablets, and 280 μl of β-mercaptoethanol at 4°C. Lysis was achieved by sonication, followed by incubation with Benzonase (20 μL of 25 unit/μL). Insoluble proteins and other cellular components were removed by centrifugation at 10,000 rpm for 60 minutes at 4°C. The supernatant was filtered using a low-protein binding 0.45 μm sterile filter. The soluble fraction was then loaded onto a Ni-NTA His-Trap FF 5 ml column (GE Healthcare). The column was washed with 20 column volumes of wash buffer (20 mM HEPES pH 7.0, 300 mM NaCl, 5% w/v glycerol, 30 mM imidazole, and 1 mM TCEP). The bound protein was eluted with 7 column volumes of elution buffer (20 mM HEPES pH 7.0, 300 mM NaCl, 5% w/v glycerol, 1 mM TCEP, and 500 mM imidazole). The collected protein was loaded onto a Hiload 26/60 Superdex 75 prep grade column (GE Healthcare) equilibrated in SEC buffer (20 mM HEPES pH 7.0, 300 mM NaCl, 5% v/v glycerol, and 1 mM TCEP). The protein eluted off the SEC column in a split peak, which was pooled into two samples and concentrated to 82 mg/mL for the first peak sample and 128 mg/mL for the second peak sample. Both samples were shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis to be >95% pure and contain a protein of the expected molecular weight. Therefore, the two samples may reflect different oligomeric states of the same protein in solution. The sample from the first peak resulted in the crystal structures, whereas the sample from the second peak yielded crystals which did not diffract to better than 3 Å resolution. The purified protein samples were stored at -80°C. Dynamic light scattering (DLS) was performed at 8 mg/mL in SEC buffer on a Malvern Instruments Nano series Zetasizer. Thermofluor experiments were performed in SEC buffer at 2 and 4 mg/mL as described .
Crystallization and structure determination
Crystallization trials were set up according to a crystallization approach  using the JCSG+ and PACT sparse matrix screens from Emerald BioSystems. Protein drops (0.4 μL at 82 mg/mL) were diluted with an equal volume of precipitant and equilibrated against 80 μL of precipitant in 96-well sitting drop vapor diffusion format using Compact Junior plates from Emerald BioSystems. A crystal grown from the JCSG+ screen condition B9 (0.1 M Na citrate pH 5.0, 20% w/v PEG 6000) was soaked into a solution containing 0.1 M Na citrate pH 5.0, 20% w/v PEG 6000, 22% v/v ethylene glycol, and 0.7 M NaI for 1 minute, then vitrified in liquid nitrogen. A data set (Table 1) was collected at 100 K under a stream of liquid nitrogen using a Rigaku FR-E+ SuperBright Cu Kα rotating anode X-ray generator with VariMax optics and a Saturn 944+ CCD detector. Data parameters include 360 images, Δφ = 1°, 2θ = 5°, 20 s exposure times, and a detector distance of 50 mm. Data were reduced with XDS . The structure was solved by combined molecular replacement and iodide ion SAD phasing. First, molecular replacement was performed using the protein model from protomer A of Clostridium thermocellum crystal structure (PDB ID 3HEE, ) as a search model in Phaser  from the CCP4 suite . Separately, twenty-one anomalous sites were identified using phenix.hyss. Phases calculated from SAD were combined with the MR solution in Phaser EP . After density improvement in parrot , the model was initially built using Buccaneer  using the default parameters for both programs and Hendrickson Lattman coefficients and Rfree rather than Phi/FOM. Another crystal of C. immitis RpiB was grown at 66 mg/mL in the presence of 20 mM ribose-5-phosphate and 12 mM MnCl2 in the PACT screen condition A2 (0.1 M SPG buffer pH 5.0, 25% w/v PEG 1500). The crystal was cryo-protected in a solution containing 20 mM ribose-5-phosphate, 12 mM MnCl2, 0.1 M SPG buffer pH 4.0, 30% w/v PEG 1500 and 20% v/v ethylene glycol and vitrified. A data set was collected as described above for the iodide crystal with the exception of 2θ = 10°, 340 images and 30 s exposure times. The phosphate bound structure was solved by molecular replacement in Phaser  using the protein-only model of the iodide phased structure. A third crystal of C. immitis RpiB was grown at 66 mg/mL in the presence of 20 mM ribose-5-phosphate and 12 mM MnCl2 in the PACT screen condition B2 (0.1 M MIB buffer pH 5.0, 25% w/v PEG 1500). The crystal was cryo-protected in a solution containing 20 mM ribose-5-phosphate, 12 mM MnCl2, 0.1 M MIB buffer pH 4.0, 30% w/v PEG 1500 and 20% v/v ethylene glycol and vitrified. A data set was collected as described above for the iodide crystal with the exception of 2θ = 10°, 360 images and 6 s exposure times. This malonic acid bound structure was solved using the protein model from the phosphate-bound structure. The final models (Table 1) were obtained after numerous rounds of refinement in REFMAC5  and manual re-building in COOT . NCS averaging was not used due to the high resolution of each structure (sub 2 Å). Both 2|Fo|-|Fc| and |Fo|-|Fc| electron density maps were used in model building. TLS refinement was used with one group per chain. Water molecules were built that were within hydrogen bonding distance to the protein (~3.2 Å) and showed electron density above 1.1 σ in the final 2|Fo|-|Fc| electron density map. Structures were assessed for correctness and validated using Molprobity . All diffraction images are freely available (http://www.csgid.org/csgid/pages/diffraction_images).
Acknowledgements and Funding
The authors thank the whole SSGCID team. This research was funded under Federal Contract Number HHSN272200700057C from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services.
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