Conservation and divergence between cytoplasmic and muscle-specific actin capping proteins: insights from the crystal structure of cytoplasmic Cap32/34 from Dictyostelium discoideum
© Eckert et al.; licensee BioMed Central Ltd. 2012
Received: 1 March 2012
Accepted: 10 May 2012
Published: 1 June 2012
Capping protein (CP), also known as CapZ in muscle cells and Cap32/34 in Dictyostelium discoideum, plays a major role in regulating actin filament dynamics. CP is a ubiquitously expressed heterodimer comprising an α- and β-subunit. It tightly binds to the fast growing end of actin filaments, thereby functioning as a “cap” by blocking the addition and loss of actin subunits. Vertebrates contain two somatic variants of CP, one being primarily found at the cell periphery of non-muscle tissues while the other is mainly localized at the Z-discs of skeletal muscles.
To elucidate structural and functional differences between cytoplasmic and sarcomercic CP variants, we have solved the atomic structure of Cap32/34 (32 = β- and 34 = α-subunit) from the cellular slime mold Dictyostelium at 2.2 Å resolution and compared it to that of chicken muscle CapZ. The two homologs display a similar overall arrangement including the attached α-subunit C-terminus (α-tentacle) and the flexible β-tentacle. Nevertheless, the structures exhibit marked differences suggesting considerable structural flexibility within the α-subunit. In the α-subunit we observed a bending motion of the β-sheet region located opposite to the position of the C-terminal β-tentacle towards the antiparallel helices that interconnect the heterodimer. Recently, a two domain twisting attributed mainly to the β-subunit has been reported. At the hinge of these two domains Cap32/34 contains an elongated and highly flexible loop, which has been reported to be important for the interaction of cytoplasmic CP with actin and might contribute to the more dynamic actin-binding of cytoplasmic compared to sarcomeric CP (CapZ).
The structure of Cap32/34 from Dictyostelium discoideum allowed a detailed analysis and comparison between the cytoplasmic and sarcomeric variants of CP. Significant structural flexibility could particularly be found within the α-subunit, a loop region in the β-subunit, and the surface of the α-globule where the amino acid differences between the cytoplasmic and sarcomeric mammalian CP are located. Hence, the crystal structure of Cap32/34 raises the possibility of different binding behaviours of the CP variants toward the barbed end of actin filaments, a feature, which might have arisen from adaptation to different environments.
KeywordsCapping protein Actin-binding Dictyostelium discoideum Structural flexibility Cap32/34 CapZ
Actin is a key component in all eukaryotic cells and plays an essential role in a wide range of cellular processes, such as migration, endocytosis, cytokinesis and generation of contraction[1–4]. Actin monomers (G-actin) are able to polymerize into filamentous actin (F-actin) resulting in polar helical structures. The two ends of the filament exhibit distinct biochemical properties and are differentiated as “barbed” and “pointed”, so named after the arrowhead appearance when filaments are decorated with myosin S1. Filament barbed ends dominate the dynamics of filament assembly due to higher association and dissociation rates for actin monomers compared to the pointed ends[3, 7, 8]. Furthermore, since the filament barbed end is preferred for actin monomer addition, whereas net disassembly is favoured at its counterpart, it is being referred to as the fast growing end (pointed end = slow growing end).
In living cells the actin cytoskeleton is in a state of rapid dynamics. Remodelling of the actin cytoskeleton is crucial in terms of inducing changes in cell shape, motility and adhesion and requires strict regulation, both temporally and spatially, thus enabling the cell to function in a controlled manner[4, 9]. This is achieved by a vast number of specialized proteins that bind to actin, thereby modulating actin filament organization and turnover in response to the changing needs of the cell[10, 11]. Actin-binding proteins are able to fulfil a large variety of tasks including the control of actin assembly and disassembly as well as regulating filament branching and bundling to help arrange actin filaments into higher order structures. They can be categorized into proteins which bind to actin monomers, filamentous actin or both. While actin monomer binding proteins control the amount and availability of monomers for polymerization, proteins that bind filamentous actin are involved, among others, in barbed and pointed end capping, filament severing, and filament crosslinking.
Capping protein (CP) is an F-actin binding protein and blocks actin filament elongation and turnover by preventing the addition of new monomers at the fast growing end. Binding of CP to actin filaments occurs with high affinity (Kd < 1 nM) and 1:1 stoichiometry. Two major variants of CP have been determined: a cytoplasmic form that is also termed Cap32/34 (32 = β- and 34 = α-subunit;) and an isoform found in the Z-discs of skeletal muscles that is often called CapZ[14, 15]. CP is a heterodimeric protein composed of an α- and a β-subunit, both having molecular masses in the range of 30–36 kDa. The protein is expressed in all eukaryotic organisms and the subunits exhibit high sequence similarity across the eukaryotic tree of life.
Vertebrates usually express three conserved isoforms of each of the α- and β-subunit[16–18] as opposed to invertebrates, plants, and lower eukaryotes, which in general contain single isoforms of each subunit. The vertebrate α-subunit isoforms are encoded by different genes, whereas the β-subunits arise by alternative splicing from a single gene[16, 17]. One isoform of both the α- and β-subunits is specifically expressed in germ cells (α3, β3), while the remaining ones (α1, α2 and β1, β2) are somatically expressed at varying ratios in different cell types and tissues. β1 is the predominant isoform in muscle cells. In contrast, β2 is mainly expressed in non-muscle tissues. The β isoforms are not able to rescue each others’ function and are thus believed to fulfil different biochemical and cellular tasks. On the other hand, there is little indication of specific functions for the α isoforms.
Vertebrates contain two somatic variants of CP. The sarcomeric variant, which is being referred to as CapZ throughout this manuscript, includes the β1 isoform and is positioned at the Z-discs of striated muscles. CapZ is proposed to help attaching actin filament barbed ends to the Z-discs and to prevent the thin filaments from growing into the adjacent sarcomere, thus serving as a key element in thin filament assembly and regulation within the Z-disc. By contrast, the cytoplasmic variant, which comprises the β2 isoform, is found at the contact sites of actin with membranes, where it is believed to play an essential role in the dendritic nucleation model. In this model activation of the Arp2/3 complex results in a branched network of actin filaments thereby generating new barbed ends, which are primarily oriented towards the cell membrane. As actin subunits are added to the newly created filament ends the membrane is pushed forward. By capping these ends over time, the growing filaments are kept short and branched, which stabilizes the filament network and sustains the propulsive force for leading edge elongation of migrating cells. In addition, actin assembly is restricted to the new barbed ends near the plasma membrane, thus enabling rapid and directed extension of the cell front.
Several molecules are able to modulate the barbed end capping activity of CP by either binding directly to the protein or through association with filament barbed ends and thereby inhibiting CP from binding. Polyphosphoinositides (PPIs), such as phosphatidylinositol-4,5-bisphosphate (PIP2)[24–26] and the proteins CARMIL and V-1 were found to directly associate with CP and to inhibit its capping activity. The crystal structures of CapZ (chicken α1/β1) in complex with CARMIL and V-1, respectively, were recently reported[29, 30]. However, to date no high resolution structure of CP bound to PIP2 exists. One possible role of PIP2, an important component of the plasma membrane and one of the most potent signalling lipids, might be to facilitate membrane movement of highly motile cells, such as those of Dictyostelium discoideum, through inhibition of actin filament capping by CP near the membrane, thus allowing rapid protrusion of the cell edge. Computational docking studies predict that PIP2 interacts with a set of three highly conserved basic residues in close proximity to the α-subunit’s C-terminus. Two of these basic residues are critical for actin filament capping. Such an interaction would therefore prevent for steric reasons the ability of CP to associate with the actin filament.
The crystal structure of CapZ (chicken α1/β1) has provided valuable insight into the atomic architecture of CP found at the Z-discs of skeletal muscles. However, until now a high-resolution structure of the cytoplasmic variant is not available. By characterizing the atomic structure of Cap32/34 from the cellular slime mold Dictyostelium discoideum as a model for cytoplasmic CP and comparing it to that of CapZ, we aimed to elucidate structural and functional differences between the two CP isoforms. This allowed us to shed light on potential interaction sites with muscle and non-muscle specific components, respectively.
Protein isolation and purification
The Dictyostelium discoideum Cap32 and Cap34 subunits were co-expressed in Escherichia coli using pETmD1-mako, an expression vector, which allows simultaneous expression of the two subunits. This vector was built on pETDuet™-1 (Novagen) by replacing the second MCS by the MCS of pDXA-mako for easy shuttling of genes between bacterial and Dictyostelium expression vectors. A full-length cDNA clone for Cap34 was obtained from the Japanese Dictyostelium cDNA project (clone VFM643;). Cap32 was assembled from two overlapping cDNA clones, SSA656 and SSJ183[35, 36]. Cap32 and Cap34 were PCR-amplified applying the Expand High Fidelity PCR System (Roche) from cDNAs using primer A (5′-GGTTATGTACAAGGTACAGAAAAGCAATTAAGTTGTTGTCTCG -3′; Cap32, forward, Bsr GI site underlined) and primer B (5′- CCGACGCGTACTACCAGCAAGATTTACTTTACCAG -3′; Cap32, reverse, Mlu I site underlined) for Cap32, and primer C (5′- CCGCCATGGCCTCAAATCAAGAATCGTTCAAATC-3′; Cap34, forward, Nco I site underlined) and primer D (5′- CCGACGCGTAAGCTTTTTTTATTTTCATTGGCAATTTTGAAGTTTTTG -3′; Cap34, reverse, Hind III site underlined) for Cap34, respectively. The PCR products were digested and subsequently ligated into pETmD1-mako. Thereby, the coding sequence of Cap32 is fused to an N-terminal 8xHis tag.
The plasmid was transformed into Escherichia coli BL21-CodonPlus(DE3)-RIL strain cells (Stratagene), which were cultured in LB broth containing 80 μg ml−1 ampicillin at 37°C until an OD(600 nm) of 0.6–0.8 was reached. Protein expression was induced at 22°C by the addition of IPTG to a final concentration of 0.1 mM and the cell culture was allowed to grow overnight. The cells were harvested by centrifugation at 6,000 X g for 15 min and the cell pellets were stored at −20°C. For protein purification the cell pellets were resuspended in 20 mM Hepes pH 7.3, 50 mM NaCl, 0.1 mM PMSF, and 1 mM DTT supplemented with EDTA-free protease-inhibitor-cocktail (Roche) and disrupted on ice by sonication. The lysate was centrifuged at 37,000 X g for 40 min to remove cellular debris.
The supernatant was sterile filtered through an Ultrafree-MC GV 0.22 μm syringe filter (Millipore) before application onto a 10 ml column of Ni-NTA superflow resin (Qiagen) pre-equilibrated with IMAC buffer (buffer A: 50 mM Hepes pH 7.3, 30 mM KAc). The column was extensively washed first with buffer A and then with 50 mM Hepes pH 7.3, 300 mM KAc (buffer B) to remove non-specifically bound proteins. The third washing step was performed with buffer A including 40 mM imidazole pH 7.3, and finally Cap32/34 was eluted from the column using a linear gradient of 40–500 mM imidazole pH 7.3 in buffer A. Fractions containing the target protein were pooled and dialyzed against 20 mM Hepes pH 7.3, 100 mM NaCl, 0,5 mM EDTA, 0.1 mM EGTA, and 1 mM MgAc. After protein concentration using a Vivaspin 6 30 k (GE Healthcare), Cap32/34 was further purified by size-exclusion chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare) equilibrated and run with 20 mM Hepes pH 7.3, 100 mM NaCl, 0,5 mM EDTA, 0.1 mM EGTA, and 1 mM MgAc.
After checking the protein purity by SDS-PAGE, the sample was concentrated to 8 mg ml−1 with a Vivaspin 6 30 k (GE Healthcare) and supplemented with sucrose to a final concentration of 3% (w/v). The protein was then divided into 50 μl aliquots in thin-walled PCR tubes, flash-cooled in liquid nitrogen, and stored at −80°C. A total of ~20 mg pure Cap32/34 was obtained from 1 L cell culture.
Crystallization, data collection, and processing
Crystallization trials were performed using hanging-drop vapor diffusion with standard sparse-matrix screens. Drops were prepared by manually dispensing 2 μl of protein solution with 2 μl reservoir solution and equilibrated against 400 μl reservoir solution in 24-well VDX plates (Hampton Research). Initial crystals formed in 100 mM Hepes pH 7.5, 20% (w/v) PEG 8000 at 20°C and grew to typical dimensions of 10 × 10 × 120 μm within 4–6 d. Subsequently, crystals were optimized by micro-seeding. The best diffracting crystals were grown in 100 mM Hepes pH 7.5, 17% (w/v) PEG 8000, and had maximum dimensions of 15 × 15 × 200 μm.
a, b, c (Å)
124.5, 124.5, 77.5
α, β, γ (º)
90, 90, 90
Resolution Range (Å)
Number of Reflections
Number of Unique Reflections
R merge †
R work ‡
R free §
Bond lengths (Å)
Bond angles (°)
Residues in most favoured regions (%)
Residues in allowed regions (%)
No. in subunit A & B
B-factor A & B (Å2)
Water (No. / B-factor)
328 / 37.9
Structure solution and refinement
Initial phases were obtained by molecular replacement (MR) using the program CNS. The structure of CapZ from Gallus gallus (PDB code 1IZN) with the solvent ions and the flexible β-subunit C-terminus (residues 252–277) omitted was used as starting model. The structural model was refined using CNS, including rigid body, simulated annealing, energy minimization, and individual B-factor refinement in several cycles. Manual inspection, rebuilding, and addition of water molecules were performed with Coot. Analysis of the Ramachandran plot reveals ~96% of the residues in most favourable regions and none in disallowed regions. The accuracy of the protein structure model was validated using MolProbity. The final model contains residues 2–272 (and 2–270 for the second molecule within the asymmetric unit, respectively) of the 281 residues of the α-subunit and all residues of the β-subunit except for residues 1, 140–145 and 251–272 (253–272 for the second molecule within the asymmetric unit). The structure was deposited in the Protein Data Bank (PDB code 4AKR). All figures were prepared with PyMOL. Structural alignments were conducted using least squares superposition (LSQ) as implemented in Coot.
Results and discussion
Overall structure of Dictyostelium discoideum Cap32/34
Cap32/34 shows the same overall architecture as CapZ
Cap32/34 reveals strong conformational flexibility in the α-subunit
Comparing the secondary structural elements of Cap32/34 and CapZ, the β-sheets in the globule regions of Cap32/34 each comprise one additional β-strand. The most prominent structural difference is located in the α-subunits in the part of the central β-sheet that is connected to the α-globule and opposite to the β-tentacle (Figure 2B). The loops connecting the β-strands move towards the antiparallel H5s giving Cap32/34 a more compact structure compared to CapZ. Based on the first crystal structure CapZ has been thought to have a fairly rigid structure except for the mobile β-tentacle. Recently and surprisingly, the structure of CapZ in complex with V-1 showed that CapZ consists of two rigid domains that undergo conformational changes but do not correspond to the two subunits. The smaller domain contains the β-globule, some β-strands of the central β-sheet, a small part of the β-H5 helix, and the α-tentacle. The crystal structure of a C-terminal truncation mutant (CapZβΔC) confirmed that CapZ has an intrinsic conformational flexibility within these two domains. The smaller domain contains the region that establishes the initial electrostatic contact with the actin-filament and conformational flexibility might therefore either prevent strong binding or be pivotal for uncapping. Here, Cap32/34 shows a different type of strong conformational flexibility that is located in the α-subunit (Figure 2B). This part is located opposite to the β-tentacle, which establishes the second actin-binding interaction. It might be important for modulating actin-binding through its influence on the tightly connected antiparallel H5 helices to which the β-tentacle is linked. Based on the structure of CapZ bound to the actin filament this region would also be ideally suited for binding CP to the membrane, either directly or mediated by another molecule. Surprisingly, in activated macrophages and platelets CP appears to be simultaneously bound to membranes and actin filaments, which would not occur if PIPs bound to the molecule that have an uncapping function. This suggests the possibility that this region of cytoplasmic CP could serve as a binding site for non-PIP lipids in motile cells, thereby mediating membrane attachment of actin. Thus, CP could have an additional role in the dendritic nucleation model apart from capping the barbed end of actin filaments.
Structure and function of the tentacles
Structure and flexibility of a linker connecting β-strands of the central β-sheet in the β-subunit
Implication of “linker βS7–S8” from Cap32 in actin-binding
In a recent NMR study of mouse cytoplasmic CP (α1/β2) interacting with the inhibitor proteins CARMIL-1 and V-1, respectively, “linker βS7–S8” was found to undergo significant chemical shift changes, suggesting that this site is involved in actin-binding[48, 49]. As part of the same study, charge reversal mutations of Lys-142 and Lys-143 severely decreased the affinity for the barbed end. In contrast, lysine to alanine mutations indicated that the residues Lys-142 and Lys-143 of mouse cytoplasmic CP (α1/β2) hardly affect actin affinity. However, mutations of the arginines and lysines of the “basic triad” already showed that substitutions by alanine only resulted in minor effects in contrast to the severe impact of the charge reversal and double/triple mutations on actin-binding. The so called “basic triad”, three highly conserved basic residues in close proximity to the CP α-subunit C-terminus, is supposed to mediate the initial contact with the barbed end of actin filaments. These residues are exposed to the solvent and thus provide the center of a basic patch on CP. Several basic and conserved residues in close proximity have also been implicated to be involved in actin-binding, namely residues R195, K223, and R225 of the β-subunit of CapZ. The “linker βS7–S8” is also in close vicinity to the “basic triad” but the two lysines are not strictly conserved and even absent in fungi and yeasts (Figure 5). Thus, we suppose that the two lysines of “linker βS7–S8”, similar to the basic residues R195, K223, and R225, are not essential for the major contact with actin, which is mediated by the “basic triad”, but contribute to the basic patch to support barbed end capping on actin-binding. In addition, the “linker βS7–S8” is located directly next to the hinge of the two rigid domains identified in CapZ that undergo conformational changes.
Cap32/34 and lipid-binding
CP is known to be inhibited by polyphosphoinositedes such as phosphatidylinositol 4,5 bisphosphate (PIP2). PIP2 does not only bind to CP but is also able to uncap CP from the barbed ends. A structure of PIP2 bound to CP is not available yet. It is known, however, from structures of other actin-binding proteins in complex with PIP2 or the sugar moieties of PIP2 that PIP2 preferentially binds to protein-specific patterns of lysines and arginines. Therefore, the region around the “basic triad” that harbours many solvent exposed lysines and arginines has been proposed to be the PIP2 binding site of CP. A triple mutation of two of the basic residues of the “basic triad” (K256 and R260) together with a closely located arginine of the β-subunit (R225) has been most effective in abolishing PIP2-binding. As in the studies of the interaction of CP with actin, single alanine mutations had been less effective compared to charge reversal, double and triple mutants. However, most of the basic residues around the “basic triad”, including βR195, βR223, βR225, and the two lysines of “linker βS7–S8”, are also conserved in all CP (Figures 4 and5) and thus could also contribute or be responsible for PIP2 binding. To unambiguously reveal the PIP2 binding site a more comprehensive mutational study or a high-resolution structure would be necessary. We also sought to characterize the structure of Cap32/34 in complex with the lipid PIP2. To accomplish that, we performed both co-crystallization and crystal soaking experiments in which the molecular ratio of the ligand was varied. Although crystals were obtained by co-crystallization there was no evidence for additional electron density. Similarly, our attempts to bind the inhibitor to Cap32/34 by soaking the crystals were not successful.
Possible interaction site of CapZ with the Z-discs of skeletal muscles
We here report the first high resolution structure of a cytoplasmic CP. The overall structure of Cap32/34 from Dictyostelium discoideum reveals a similar arrangement as compared to its sarcomeric variant CapZ. Like in CapZ, the individual CP subunits exhibit very similar secondary and tertiary structures despite sharing a very low sequence homology. Moreover, the subunits are extensively intertwined and organized in such a way that the molecule has a pseudo 2-fold axis of rotational symmetry down its center point.
As has been observed in CapZ, the Cap32/34 structure showed the attachment of the α-tentacle to the central β-sheet and the antiparallel H5 helices, and supported the highly flexible nature of the β-tentacle, which is proposed to swing out and bind to actin. By superposition onto CapZ we observed considerably structural flexibility in the α-subunits. In Cap34 the region located opposite to the C-terminal β-tentacle moves towards the antiparallel helices that interconnect the heterodimer leading to a more compact CP structure. This bending motion demonstrates additional flexibility in CP to the two domain twisting attributed mainly to the β-subunit as observed in the structure of CapZ complexed with V-1.
Furthermore, there is evidence that, in terms of cytoplasmic CP, an additional protein segment might be important for mediating high affinity capping of actin filaments. Based on the crystal structure of Cap32/34, the molecule comprises a dynamic loop region located between S7 and S8 within its β-subunit, denoted here as “linker βS7–S8”, which has recently been reported to be important for the association of cytoplasmic CP with actin. This observation is in marked contrast to CapZ, in which the corresponding region has been found to be well ordered. Since “linker βS7–S8” provides a positively charged surface close to the basic patch on CP, it might participate in the initial electrostatic binding to acidic regions on the barbed end of actin filaments.
Finally, to date information about potential interaction sites of CapZ with the Z-disc of the sarcomere is not available. By comparing the structures of the two CP variants, we were able to detect a solvent-exposed region within the CapZ α-subunit (residues Leu-101 – Leu-117 located in the α-globule), greatly differing in both conformation and flexibility from that of Cap32/34. We therefore hypothesize that this protein segment might be involved in the binding of CapZ to the Z-disc in muscle cells.
- Dd :
- Gg :
Root mean square deviation
- Sc :
We would like to thank Elspeth Gordon and David Flot at ESRF (European Synchrotron Radiation Facility) microfocus beamline ID 23-2 for their help with data collection, in particular the ‘helical’ data collection. We highly appreciate the Dictyostelium cDNA project in Japan and the Japanese National BioResource Project for providing the cDNA clones. This work has been funded by grants KO 2251/3-1, KO 2251/3-2, and KO 2251/3-3 of the Deutsche Forschungsgemeinschaft.
- Pollard TD, Borisy GG: Cellular motility driven by assembly and disassembly of actin filaments. Cell 2003, 112: 453–465. 10.1016/S0092-8674(03)00120-XView ArticlePubMed
- Galletta BJ, Cooper JA: Actin and endocytosis: mechanisms and phylogeny. Curr Opin Cell Biol 2009, 21: 20–27. 10.1016/j.ceb.2009.01.006PubMed CentralView ArticlePubMed
- Bugyi B, Carlier MF: Control of actin filament treadmilling in cell motility. Annu Rev Biophys 2010, 39: 449–470. 10.1146/annurev-biophys-051309-103849View ArticlePubMed
- Firat-Karalar EN, Welch MD: New mechanisms and functions of actin nucleation. Curr Opin Cell Biol 2011, 23: 4–13. 10.1016/j.ceb.2010.10.007PubMed CentralView ArticlePubMed
- Holmes KC: Structural biology: actin in a twist. Nature 2009, 457: 389–390. 10.1038/457389aView ArticlePubMed
- Moore PB, Huxley HE, DeRosier DJ: Three-dimensional reconstruction of F-actin, thin filaments and decorated thin filaments. J Mol Biol 1970, 50: 279–295. 10.1016/0022-2836(70)90192-0View ArticlePubMed
- Kueh HY, Mitchison TJ: Structural plasticity in actin and tubulin polymer dynamics. Science 2009, 325: 960–963. 10.1126/science.1168823PubMed CentralView ArticlePubMed
- Pantaloni D, Le Clainche C, Carlier MF: Mechanism of actin-based motility. Science 2001, 292: 1502–1506. 10.1126/science.1059975View ArticlePubMed
- Dominguez R: Structural insights into de novo actin polymerization. Curr Opin Struct Biol 2010, 20: 217–225. 10.1016/j.sbi.2009.12.012PubMed CentralView ArticlePubMed
- Winder SJ, Ayscough KR: Actin-binding proteins. J Cell Sci 2005, 118: 651–654. 10.1242/jcs.01670View ArticlePubMed
- Cooper JA, Sept D: New insights into mechanism and regulation of actin capping protein. Int Rev Cell Mol Biol 2008, 267: 183–206.PubMed CentralView ArticlePubMed
- Le Clainche C, Carlier MF: Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol Rev 2008, 88: 489–513. 10.1152/physrev.00021.2007View ArticlePubMed
- Hartmann H, Schleicher M, Noegel AA: Heterodimeric capping proteins constitute a highly conserved group of actin-binding proteins. Dev Genet 1990, 11: 369–376. 10.1002/dvg.1020110508View ArticlePubMed
- Casella JF, Craig SW, Maack DJ, Brown AE: Cap Z(36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle. J Cell Biol 1987, 105: 371–379. 10.1083/jcb.105.1.371View ArticlePubMed
- Caldwell JE, Heiss SG, Mermall V, Cooper JA: Effects of CapZ, an actin capping protein of muscle, on the polymerization of actin. Biochemistry 1989, 28: 8506–8514. 10.1021/bi00447a036View ArticlePubMed
- von Bulow M, Rackwitz HR, Zimbelmann R, Franke WW: CP beta3, a novel isoform of an actin-binding protein, is a component of the cytoskeletal calyx of the mammalian sperm head. Exp Cell Res 1997, 233: 216–224. 10.1006/excr.1997.3564View ArticlePubMed
- Schafer DA, Korshunova YO, Schroer TA, Cooper JA: Differential localization and sequence analysis of capping protein beta-subunit isoforms of vertebrates. J Cell Biol 1994, 127: 453–465. 10.1083/jcb.127.2.453View ArticlePubMed
- Hart MC, Korshunova YO, Cooper JA: Mapping of the mouse actin capping protein alpha subunit genes and pseudogenes. Genomics 1997, 39: 264–270. 10.1006/geno.1996.4506View ArticlePubMed
- Hart MC, Korshunova YO, Cooper JA: Vertebrates have conserved capping protein alpha isoforms with specific expression patterns. Cell Motil Cytoskelet 1997, 38: 120–132. 10.1002/(SICI)1097-0169(1997)38:2<120::AID-CM2>3.0.CO;2-BView Article
- Hart MC, Cooper JA: Vertebrate isoforms of actin capping protein beta have distinct functions in vivo. J Cell Biol 1999, 147: 1287–1298. 10.1083/jcb.147.6.1287PubMed CentralView ArticlePubMed
- Amatruda JF, Cooper JA: Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein. J Cell Biol 1992, 117: 1067–1076. 10.1083/jcb.117.5.1067View ArticlePubMed
- Mullins RD, Heuser JA, Pollard TD: The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments. Proc Natl Acad Sci U S A 1998, 95: 6181–6186. 10.1073/pnas.95.11.6181PubMed CentralView ArticlePubMed
- Carlier MF, Pantaloni D: Control of actin dynamics in cell motility. J Mol Biol 1997, 269: 459–467. 10.1006/jmbi.1997.1062View ArticlePubMed
- Haus U, Hartmann H, Trommler P, Noegel AA, Schleicher M: F-actin capping by cap32/34 requires heterodimeric conformation and can be inhibited with PIP2. Biochem Biophys Res Commun 1991, 181: 833–839. 10.1016/0006-291X(91)91265-EView ArticlePubMed
- Kim K, McCully ME, Bhattacharya N, Butler B, Sept D, Cooper JA: Structure/function analysis of the interaction of phosphatidylinositol 4,5-bisphosphate with actin-capping protein: implications for how capping protein binds the actin filament. J Biol Chem 2007, 282: 5871–5879.PubMed CentralView ArticlePubMed
- Kuhn JR, Pollard TD: Single molecule kinetic analysis of actin filament capping. Polyphosphoinositides do not dissociate capping proteins. J Biol Chem 2007, 282: 28014–28024. 10.1074/jbc.M705287200View ArticlePubMed
- Remmert K, Olszewski TE, Bowers MB, Dimitrova M, Ginsburg A, Hammer JA: CARMIL is a bona fide capping protein interactant. J Biol Chem 2004, 279: 3068–3077.View ArticlePubMed
- Taoka M, Ichimura T, Wakamiya-Tsuruta A, Kubota Y, Araki T, Obinata T, Isobe T: V-1, a protein expressed transiently during murine cerebellar development, regulates actin polymerization via interaction with capping protein. J Biol Chem 2003, 278: 5864–5870. 10.1074/jbc.M211509200View ArticlePubMed
- Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC: Structural characterization of a capping protein interaction motif defines a family of actin filament regulators. Nat Struct Mol Biol 2010, 17: 497–503. 10.1038/nsmb.1792PubMed CentralView ArticlePubMed
- Takeda S, Minakata S, Koike R, Kawahata I, Narita A, Kitazawa M, Ota M, Yamakuni T, Maeda Y, Nitanai Y: Two distinct mechanisms for actin capping protein regulation—steric and allosteric inhibition. PLoS Biol 2010, 8: e1000416. 10.1371/journal.pbio.1000416PubMed CentralView ArticlePubMed
- Narita A, Takeda S, Yamashita A, Maeda Y: Structural basis of actin filament capping at the barbed-end: a cryo-electron microscopy study. EMBO J 2006, 25: 5626–5633. 10.1038/sj.emboj.7601395PubMed CentralView ArticlePubMed
- Yamashita A, Maeda K, Maeda Y: Crystal structure of CapZ: structural basis for actin filament barbed end capping. EMBO J 2003, 22: 1529–1538. 10.1093/emboj/cdg167PubMed CentralView ArticlePubMed
- Kollmar M: Use of the myosin motor domain as large-affinity tag for the expression and purification of proteins in Dictyostelium discoideum. Int J Biol Macromol 2006, 39: 37–44. 10.1016/j.ijbiomac.2006.01.005View ArticlePubMed
- Urushihara H, Morio T, Tanaka Y: The cDNA sequencing project. Methods Mol Biol 2006, 346: 31–49.PubMed
- Urushihara H, Morio T, Saito T, Kohara Y, Koriki E, Ochiai H, Maeda M, Williams JG, Takeuchi I, Tanaka Y: Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum. Nucleic Acids Res 2004, 32: 1647–1653. 10.1093/nar/gkh262PubMed CentralView ArticlePubMed
- Morio T, Urushihara H, Saito T, Ugawa Y, Mizuno H, Yoshida M, Yoshino R, Mitra BN, Pi M, Sato T, et al.: The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res 1998, 5: 335–340. 10.1093/dnares/5.6.335View ArticlePubMed
- Flot D, Mairs T, Giraud T, Guijarro M, Lesourd M, Rey V, van Brussel D, Morawe C, Borel C, Hignette O, et al.: The ID23–2 structural biology microfocus beamline at the ESRF. J Synchrotron Radiat 2010, 17: 107–118. 10.1107/S0909049509041168PubMed CentralView ArticlePubMed
- Kabsch W: Xds. Acta Crystallogr D: Biol Crystallogr 2010, 66: 125–132. 10.1107/S0907444909047337View Article
- Kabsch W: Integration, scaling, space-group assignment and post-refinement. Acta Crystallogr D: Biol Crystallogr 2010, 66: 133–144. 10.1107/S0907444909047374View Article
- Brunger AT: Version 1.2 of the crystallography and NMR system. Nat Protoc 2007, 2: 2728–2733. 10.1038/nprot.2007.406View ArticlePubMed
- Emsley P, Cowtan K: Coot: model-building tools for molecular graphics. Acta Crystallogr D: Biol Crystallogr 2004, 60: 2126–2132. 10.1107/S0907444904019158View Article
- Davis IW, Leaver-Fay A, Chen VB, Block JN, Kapral GJ, Wang X, Murray LW, Arendall WB, Snoeyink J, Richardson JS, et al.: MolProbity: all-atom contacts and structure validation for proteins and nucleic acids. Nucleic Acids Res 2007, 35: W375-W383. 10.1093/nar/gkm216PubMed CentralView ArticlePubMed
- DeLano Scientific. http://www.pymol.org
- Odronitz F, Kollmar M: Pfarao: a web application for protein family analysis customized for cytoskeletal and motor proteins (CyMoBase). BMC Genomics 2006, 7: 300. 10.1186/1471-2164-7-300PubMed CentralView ArticlePubMed
- CyMoBase—a database for cytoskeletal and motor protein sequence information. http://www.cymobase.org
- Talavera G, Castresana J: Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol 2007, 56: 564–577. 10.1080/10635150701472164View ArticlePubMed
- Hartwig JH, Bokoch GM, Carpenter CL, Janmey PA, Taylor LA, Toker A, Stossel TP: Thrombin receptor ligation and activated Rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets. Cell 1995, 82: 643–653. 10.1016/0092-8674(95)90036-5View ArticlePubMed
- Zwolak A, Uruno T, Piszczek G, Hammer JA, Tjandra N: Molecular basis for barbed end uncapping by CARMIL homology domain 3 of mouse CARMIL-1. J Biol Chem 2010, 285: 29014–29026. 10.1074/jbc.M110.134221PubMed CentralView ArticlePubMed
- Zwolak A, Fujiwara I, Hammer JA, Tjandra N: Structural basis for capping protein sequestration by myotrophin (V-1). J Biol Chem 2010, 285: 25767–25781. 10.1074/jbc.M110.135848PubMed CentralView ArticlePubMed
- Kim T, Cooper JA, Sept D: The interaction of capping protein with the barbed end of the actin filament. J Mol Biol 2010, 404: 794–802. 10.1016/j.jmb.2010.10.017PubMed CentralView ArticlePubMed
- Kim K, Yamashita A, Wear MA, Maeda Y, Cooper JA: Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. J Cell Biol 2004, 164: 567–580. 10.1083/jcb.200308061PubMed CentralView ArticlePubMed
- Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA: How capping protein binds the barbed end of the actin filament. Curr Biol 2003, 13: 1531–1537. 10.1016/S0960-9822(03)00559-1View ArticlePubMed
- Bhattacharya N, Ghosh S, Sept D, Cooper JA: Binding of myotrophin/V-1 to actin-capping protein: implications for how capping protein binds to the filament barbed end. J Biol Chem 2006, 281: 31021–31030. 10.1074/jbc.M606278200PubMed CentralView ArticlePubMed
- Huang S, Gao L, Blanchoin L, Staiger CJ: Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid. Mol Biol Cell 2006, 17: 1946–1958. 10.1091/mbc.E05-09-0840PubMed CentralView ArticlePubMed
- Schafer DA, Jennings PB, Cooper JA: Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides. J Cell Biol 1996, 135: 169–179. 10.1083/jcb.135.1.169View ArticlePubMed
- Pappas CT, Bhattacharya N, Cooper JA, Gregorio CC: Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc. Mol Biol Cell 2008, 19: 1837–1847. 10.1091/mbc.E07-07-0690PubMed CentralView ArticlePubMed