Structural analysis of the carboxy terminal PH domain of pleckstrin bound to D-myo-inositol 1,2,3,5,6-pentakisphosphate
© Jackson et al; licensee BioMed Central Ltd. 2007
Received: 17 May 2007
Accepted: 22 November 2007
Published: 22 November 2007
Pleckstrin homology (PH) domains are one of the most prevalent domains in the human proteome and represent the major phosphoinositide-binding module. These domains are often found in signaling proteins and function predominately by targeting their host proteins to the cell membrane. Inositol phosphates, which are structurally similar to phosphoinositides, are not only known to play a role as signaling molecules but are also capable of being bound by PH domains.
In the work presented here it is shown that the addition of commercial myo-inositol hexakisphosphate (IP6) inhibited the binding of the carboxy terminal PH domain of pleckstrin (C-PH) to phosphatidylinositol 3,4-bisphosphate with an IC50 of 7.5 μM. In an attempt to characterize this binding structurally, C-PH was crystallized in the presence of IP6 and the structure was determined to 1.35 Å. Examination of the resulting electron density unexpectedly revealed the bound ligand to be D-myo-inositol 1,2,3,5,6-pentakisphosphate.
The discovery of D-myo-inositol 1,2,3,5,6-pentakisphosphate in the crystal structure suggests that the inhibitory effects observed in the binding studies may be due to this ligand rather than IP6. Analysis of the protein-ligand interaction demonstrated that this myo-inositol pentakisphosphate isomer interacts specifically with protein residues known to be involved in phosphoinositide binding. In addition to this, a structural alignment of other PH domains bound to inositol phosphates containing either four or five phosphate groups revealed that the majority of phosphate groups occupy conserved locations in the binding pockets of PH domains. These findings, taken together with other recently reported studies suggest that myo- inositol pentakisphosphates could act to regulate PH domain-phosphoinositide interactions by directly competing for binding, thus playing an important role as signaling molecules.
Pleckstrin is an intriguing platelet protein that appears to be involved in reorganization of the cytoskeleton, as well as attenuating various signaling pathways following platelet activation [1–6]. On cloning pleckstrin, two internal repeats consisting of approximately 100 amino acids including 30 identical residues were identified at the N- and C-termini of the protein [7, 8]. Similar regions were later recognized in other proteins and these internal repeats were consequently termed pleckstrin homology (PH) domains [9, 10].
Since their first identification in pleckstrin, PH domains have been found in over four hundred human proteins (SMART database,) making this domain one of the most common in the human proteome. PH domain-containing proteins are known to be involved in a number of different cellular functions, including phosphoinositide metabolism, protein phosphorylation and cytoskeletal organization, suggesting that PH domains themselves may also function in a variety of different ways (reviewed in [12, 13]). The crystal and solution structures of numerous PH domains have been determined revealing that despite sharing only low sequence similarity, they maintain a highly conserved fold. The domain structure consists of a seven-stranded anti-parallel β-sandwich that is closed at one end by a C-terminal alpha helix and remains open at the other end, where several variable loop regions are located (reviewed in ). Traditionally, PH domains have been thought to function predominately as phosphoinositide-binding modules, targeting their host proteins to the membrane where they can carry out their various functions. The region known to bind these signaling lipids has been identified as the variable β1–β2 loop, with some PH domains contributing additional interacting residues from nearby secondary structure elements. Despite their early characterization as phosphoinositide-binding modules, it is now clear that the majority of PH domains do not bind phosphoinositides with sufficient affinity or specificity to drive membrane localization , suggesting that alternate functions are likely to exist for these domains. In support of this notion, several reports have shown that within some proteins, PH domains function to mediate protein-protein interactions [14–16].
Given their wide distribution, it is likely that PH domains mediate other processes in addition to protein-protein interactions and membrane association through phosphoinositide-specific binding. In particular, it seems possible that specific soluble inositol phosphates (such as inositol 1,4,5-trisphosphate[IP3]), could bind to PH domains and thereby serve as important regulators . Inositol phosphates are structurally very similar to the phosphoinositide head groups known to bind to many PH domains. In fact, it has been well established that PH domains can bind inositol phosphates in vitro and have been used extensively in structural and biochemical studies focused on understanding phosphoinositide-PH domain interactions. Despite this they have received relatively little consideration as physiological PH domain ligands.
Previous studies have shown that phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P2] binds to C-PH much more firmly than other phosphoinositides [18, 19] and that IP6 is a potent inhibitor of pleckstrin-phosphoinositide interactions . Based on these findings, we found that commercial IP6 also competed effectively with PtdIns(3,4)P2 for binding to C-PH. As we have recently reported the crystal structure of C-PH in the unliganded form , we then set out to determine the structural basis for the interaction with IP6. To our surprise, we found that the complex formed contained D-myo-inositol 1,2,3,5,6-pentakisphosphate [Ins(1,2,3,5,6)P5], rather than IP6. The structure presented here indicates that inositol polyphosphates can interact specifically with PH domains and therefore lends further support to the idea that at least some inositol phosphates may regulate PH domain phosphoinositide association.
Results and Discussion
Addition of IP6effectively competes for binding of PtdIns(3,4)P2to C-PH
Structure of C-PH/ligand complex
Crystallographic and Data Refinement Statistics
Unit-cell parameters (Å)
a = 32.2, b = 47.7 and c = 64.1
α = β = γ = 90
No. of molecules in asymmetric unit
Resolution range (Å) a
32.03 – 1.35 (1.40–1.35)
Data Redundancy a
R merge (%) a
Model and refinement
Resolution range (Å) a
38.26 – 1.35 (1.39–1.35)
R work (%)
R free (%)
No. of reflections
20 722 (19 638 in working set and 1084 in test set)
No. of amino acid residues/atoms
No. of waters
r.m.s.d bond lengths (Å)
r.m.s.d bond angles (°)
Average B factor (Å 2 )
Analysis of the C-PH/Ins(1,2,3,5,6)P5Complex
Comparison of the C-PH/Ins(1,2,3,5,6)P5and Grp1/Ins(1,3,4,5,6)P5structures
The second overlapping phosphate position is occupied by the 6 and 1-phosphates of Ins(1,2,3,5,6)P5 and Ins(1,3,4,5,6)P5, respectively. In the C-PH structure, the 6-phosphate interacts with R258, a residue that is not conserved in the Grp1 PH domain. The corresponding 1-phosphate of Ins(1,3,4,5,6)P5 interacts with K282 in the Grp1 PH domain which is equivalent to K262 in C-PH, although this residue does not interact with the 6-phosphate of Ins(1,2,3,5,6)P5 due to the location of its side chain. The 5-phosphate of Ins(1,2,3,5,6)P5 and the 6-phosphate of Ins(1,3,4,5,6)P5 occupy the third overlapping position within the phosphoinositide-binding pocket. The 5-phosphate is in position to interact with the main chains of H256, R257 and N260 as well as the side chain of R257 in C-PH. Although this arginine is conserved in the Grp1 PH domain (R277), it does not interact with the 6-phosphate, in fact the 6-phosphate makes less optimal interactions with main chain residues compared to those observed in the C-PH structure. In the fourth and final overlapping position, the 3-phosphate of Ins(1,2,3,5,6)P5 and the 4-phosphate of Ins(1,3,4,5,6)P5 both interact with a conserved tyrosine at position 277 in C-PH and 295 in the Grp1 PH domain. In addition to this, the 4-phosphate makes further stabilizing contacts with the side chains of H355 and K273, interactions that are not observed in the C-PH/Ins(1,2,3,5,6)P5 structure.
D-myo-inositol pentakisphosphates as signaling molecules
The physiological significance of the interaction reported here with respect to platelet signaling pathways is currently unknown and calls for further careful examination. With the exception of IP3, inositol phosphates have only been thought to function with minor roles as second messengers . However, as more information becomes available it appears as though these molecules have a much broader role in signaling then was originally appreciated [26, 27]. The most well-characterized inositol phosphate second messenger is IP3, produced by phospholipase C-mediated hydrolysis of PtdIns(4,5)P2. In addition to IP3, it has also been proposed that Ins(1,3,4,5)P4 plays a role in the regulation of cellular Ca+2 fluxes . The IP5 family of D-myo-inositol pentakisphosphates represents one of the most abundant forms of inositol phosphates present in mammalian cells. Although six possible isomers exist, Ins(1,3,4,5,6)P5 represents the predominant form observed in mammalian cells, including platelets [27–30]. Other IP5 isomers including Ins(1,2,4,5,6)P5 and Ins(2,3,4,5,6)P5 have been detected in a number of cell lines [29, 31], demonstrating that these molecules are indeed present in mammalian cells and as such could play roles in regulating biological functions. In agreement with this, several recent studies have provided evidence suggesting specific roles for IP5 isomers ranging from the regulation of chromatin remodeling [32, 33] to Salmonella pathogenesis [34, 35]. In further support of this idea, Ins(1,3,4,5,6)P5 was recently shown to be capable of promoting apoptosis and to possess antiangiogenic and antitumor effects as a result of its ability to inhibit the phosphoinositide 3-kinase/Akt signaling pathway [36, 37]. This inhibition was shown to be a consequence of Ins(1,3,4,5,6)P5 competing with PtdIns(3,4,5)P3 for binding to the protein kinase B (Akt) PH domain, ultimately preventing the phosphorylation and activation of Akt. Based on these intriguing results the authors have proposed that Ins(1,3,4,5,6)P5 and potentially other inositol phosphates can be used as experimental or possibly therapeutic tools that interfere with the binding of PH domains involved in signaling pathways. Inositol pyrophosphates, specifically IP7 and IP8, have also been shown to act as signaling molecules that regulate PH domain interactions with phosphoinositides . In this study IP7 and IP8 were shown to compete with PtdIns(3,4,5)P3 for binding to several PH domain-containing proteins in Dictyostelium, resulting in an effect on chemotaxis. As the body of evidence continues to grow, it appears that PH domains will emerge as versatile domains capable of mediating interactions with a range of different ligands.
It therefore seems likely that as more studies are aimed at examining the roles of IP5 isomers, it will become apparent that this family of inositol phosphates is involved in signaling pathways that affect a range of physiological processes. The identification of PH domains capable of interacting specifically with the various IP5 isomers will aid in characterizing the roles of the latter. However, we are not aware of any report of the presence of Ins(1,2,3,5,6)P5 in platelets or other cells at the present time. It will therefore be important to investigate the effects of other IP5 isomers on the binding of pleckstrin C-PH domain to PtdIns(3,4)P2. It has not escaped our attention that in addition to acting as antagonists of pleckstrin binding by specific phosphoinositides, myo- inositol polyphosphates with high affinities for C-PH could act as regulators of the interaction of pleckstrin with other proteins.
In the work presented here, the addition of commercial IP6 was shown to compete with PtdIns(3,4)P2 for binding to C-PH. Since Ins(1,2,3,5,6)P5 was found bound to C-PH in the crystal structure it would appear that the inhibitory effects observed in the binding studies were due to contamination of IP6 with Ins(1,2,3,5,6)P5, which binds to C-PH with a higher affinity. Regardless of its source, Ins(1,2,3,5,6)P5 binds specifically in the phosphoinositide binding cleft of C-PH making numerous interactions with residues known to be involved in binding PtdIns(3,4)P2. In a structural comparison with the Grp1 PH domain bound to Ins(1,3,4,5,6)P5 it was observed that despite differences in their arrangement about the inositol ring, four out of the five phosphate groups from these two ligands occupy conserved positions. This structural analysis, in combination with other recently published data discussed above, suggests that myo-inositol pentakisphosphates could act to regulate PH domain-phosphoinositide interactions by directly competing for binding to these domains. It is also possible that myo-inositol pentakisphosphates could play roles in signaling pathways by acting as inducers of protein-protein interactions.
C-PH binding assays
The binding assays were performed as described in . Briefly, 0.3 μM recombinant C-PH was incubated with large unilamellar vesicles (LUVs) consisting of phosphatidylcholine supplemented with 5 mol% PtdIns(3,4)P2. Simultaneous additions of IP6 (Aldrich) (0–100 μM) were used to inhibit the binding of C-PH to the PtdIns(3,4)P2. LUVs with bound protein were isolated by ultracentrifugation, analyzed by SDS-PAGE using 10% acrylamide and immunoblotted with antibody to the C-terminal 13 residues of pleckstrin. A logit plot was used to calculate the apparent IC50 of IP6 (y = 100[1 + (x/IC50)s]-1, where y is the % of added C-PH bound at the inhibitor concentration x and s is a slope factor).
Protein expression and purification
C-PH (pleckstrin amino acids 240–350) used for LUV binding studies was expressed and purified as described previously [19, 20]. For crystallography, C- and N-terminal residues previously found to lack structure  were omitted. Thus, the carboxy terminal PH domain (amino acids 243–347) of human pleckstrin was expressed and purified as a hexahistidine fusion protein in E. coli BL21(DE3) cells using the pDEST17 expression vector (Invitrogen). Cells were grown in standard LB medium supplemented with 10 mg ml-1 ampicillin at 310 K with shaking (225 rev min-1) until the light absorbance at 600 nm reached 0.5. Protein expression was then induced using 0.1 mM IPTG and the incubation temperature was lowered to 300 K. After a 5-hour induction period cells were harvested by centrifugation at 4 000 rpm for 10 minutes at a temperature of 277 K. Each 1L cell pellet was then resuspended in 8 ml of 1× phosphate buffered saline (PBS) and centrifuged at 3 500 rpm for 10 minutes at 277 K. The resulting cell pellets were then frozen in liquid nitrogen and stored at 193 K. Prior to cell lyses using high pressure, cell pellets (2 L) were resuspended to a final volume of 35 ml using nickel A buffer (20 mM Hepes pH 7.5, 0.5 M NaCl, 0.06% LDAO, 2 mM β-mercaptoethanol and 10 mM imidazole). Following cell lyses samples were centrifuged at 20 000 rpm for 40 minutes at 277 K and the resulting supernatant was applied to a HiTrap Nickel affinity column (Amersham Biosciences). The C-PH was eluted from the column with 210 mM imidazole following sequential washes with 15 and 30 mM imidazole which was accomplished by mixing appropriate volumes of nickel A buffer and nickel B buffer (20 mM Hepes pH 7.5, 0.5 M NaCl, 0.06% LDAO, 2 mM β-mercaptoethanol and 300 mM imidazole). The protein sample was then diluted in S-A buffer (10 mM Hepes pH 7.5, 1 mM dithiothreitol and 1 mM EDTA) to lower the salt concentration to approximately 75 mM. This sample was then applied to a HiTrap SP Sepharose HP ion exchange column (Amersham Biosciences) to further purify the protein from any remaining contaminants. The C-PH was subsequently eluted using a gradient of increasing salt concentration generated by the application of S-B buffer (10 mM Hepes pH 7.5, 1 mM dithiothreitol, 1 mM EDTA and 0.5 M NaCl). The hexahistidine tag was removed from the C-PH by cleavage with TEV protease. This results in four residues (Gly, Ser, Phe and Thr) being left N-terminal to the first residue in C-PH. Of these four amino acids only the phenylalanine and threonine residues could be reliably built into the available electron density. In order to purify C-PH from the cleaved hexahistidine tag and the TEV protease the digested sample was re-applied to the HiTrap SP Sepharose column and purified as described above. The resulting C-PH sample was buffer exchanged into the final crystallization buffer (5 mM pottasium glutamate, 120 mM sodium glutamate, 20 mM Hepes pH 7.5, 2.5 mM EDTA, 2.5 mM EGTA and 3.15 mM MgCl2) using a HiPrep 26/10 Desalting column (Amersham Biosciences). The resulting protein sample was concentrated to 4.0 mg ml-1 as determined by the Bradford assay, using a centrifugal filter. SDS-PAGE analysis indicates that C-PH prepared by this method is greater then 95% pure.
Crystallization and data collection of the C-PH/Ins(1,2,3,5,6)P5complex
C-PH (2.5 mg ml-1) was crystallized in the presence of 1 mM IP6 (Aldrich) using the hanging drop vapour diffusion method under the following conditions. A 2 μl drop containing 1 μl of C-PH (2.5 mg ml-1) and 1 mM IP6 in 5 mM potassium glutamate, 120 mM sodium glutamate, 20 mM Hepes pH 7.5, 2.5 mM EDTA, 2.5 mM EGTA and 3.15 mM MgCl2 and 1 μl of 0.1 M Bis-Tris pH 6.5 and 28% polyethylene glycol 2 000 monomethyl ether was suspended over 500 μl of 0.75 M ammonium sulfate and incubated at 293 K. Rectangular rod-shaped crystals grown in these conditions were harvested and crushed to generate micronuclei that were subsequently used to streak seed into the same crystallization condition as well as slight variations of this condition. This resulted in large rectangular rod-shaped crystals with dimensions of approximately 400 × 100 × 100 μm. The crystal used to collect data was grown in 0.1 M Bis-Tris pH 6.5 and 28% polyethylene glycol 2 000 monomethyl ether. Prior to cryocooling in a nitrogen cold stream, the crystal was soaked for approximately 10 seconds in a cryoprotectant containing 0.1 M Bis-Tris pH 6.5, 28% polyethylene glycol 2 000 monomethyl ether and 17% glycerol. Both high (1.35 Å) and low (1.5 Å) resolution data sets were collected independently for this crystal and then merged to yield a single data set. The data was collected at a wavelength of 1.0 Å at beamline X25 of the Brookhaven National Laboratory using a ADSC Q315 CCD x-ray detector and processed using the HKL2000 program suite .
Structure determination and model refinement
The crystal structure of the C-PH in complex with Ins(1,2,3,5,6)P5 was solved by molecular replacement using the program MOLREP . The search model used in molecular replacement was the crystal structure of the C-PH (PDB code 1ZM0), which was solved in the absence of any ligand. Iterative cycles of model building and refinement were carried out using the programs WinCoot  and Refmac5 [42, 43] respectively. All figures describing protein structures presented were generated using the molecular graphics program PyMol .
We wish to thank Dr. Kalinka Koteva for performing HPLC analysis to assess the purity of IP6. SGJ is a recipient of a CIHR Canada Graduate Scholarship Master's Award. This work was supported by independent grants to RJH and MSJ from CIHR.
- Abrams CS, Wu H, Zhao W, Belmonte E, White D, Brass LF: Pleckstrin inhibits phosphoinositide hydrolysis initiated by G-protein-coupled and growth factor receptors. A role for pleckstrin's PH domains. J Biol Chem 1995, 270(24):14485–14492. 10.1074/jbc.270.24.14485View ArticlePubMedGoogle Scholar
- Abrams CS, Zhang J, Downes CP, Tang X, Zhao W, Rittenhouse SE: Phosphopleckstrin inhibits gbetagamma-activable platelet phosphatidylinositol-4,5-bisphosphate 3-kinase. J Biol Chem 1996, 271(41):25192–25197. 10.1074/jbc.271.41.25192View ArticlePubMedGoogle Scholar
- Auethavekiat V, Abrams CS, Majerus PW: Phosphorylation of platelet pleckstrin activates inositol polyphosphate 5-phosphatase I. J Biol Chem 1997, 272(3):1786–1790. 10.1074/jbc.272.3.1786View ArticlePubMedGoogle Scholar
- Ma AD, Brass LF, Abrams CS: Pleckstrin associates with plasma membranes and induces the formation of membrane projections: requirements for phosphorylation and the NH2-terminal PH domain. J Cell Biol 1997, 136(5):1071–1079. 10.1083/jcb.136.5.1071PubMed CentralView ArticlePubMedGoogle Scholar
- Ma AD, Abrams CS: Pleckstrin induces cytoskeletal reorganization via a Rac-dependent pathway. J Biol Chem 1999, 274(40):28730–28735. 10.1074/jbc.274.40.28730View ArticlePubMedGoogle Scholar
- Roll RL, Bauman EM, Bennett JS, Abrams CS: Phosphorylated pleckstrin induces cell spreading via an integrin-dependent pathway. J Cell Biol 2000, 150(6):1461–1466. 10.1083/jcb.150.6.1461PubMed CentralView ArticlePubMedGoogle Scholar
- Tyers M, Rachubinski RA, Stewart MI, Varrichio AM, Shorr RG, Haslam RJ, Harley CB: Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature 1988, 333(6172):470–473. 10.1038/333470a0View ArticlePubMedGoogle Scholar
- Tyers M, Haslam RJ, Rachubinski RA, Harley CB: Molecular analysis of pleckstrin: the major protein kinase C substrate of platelets. J Cell Biochem 1989, 40(2):133–145. 10.1002/jcb.240400202View ArticlePubMedGoogle Scholar
- Haslam RJ, Koide HB, Hemmings BA: Pleckstrin domain homology. Nature 1993, 363(6427):309–310. 10.1038/363309b0View ArticlePubMedGoogle Scholar
- Mayer BJ, Ren R, Clark KL, Baltimore D: A putative modular domain present in diverse signaling proteins. Cell 1993, 73(4):629–630. 10.1016/0092-8674(93)90244-KView ArticlePubMedGoogle Scholar
- Letunic I, Copley RR, Pils B, Pinkert S, Schultz J, Bork P: SMART 5: domains in the context of genomes and networks. Nucleic Acids Res 2006, (34 Database):D257–60. 10.1093/nar/gkj079
- Lemmon MA: Pleckstrin homology domains : not just for phosphoinositides. Biochem Soc Trans 2004, 32(Pt 5):707–711.View ArticlePubMedGoogle Scholar
- Cozier GE, Carlton J, Bouyoucef D, Cullen PJ: Membrane targeting by pleckstrin homology domains. Curr Top Microbiol Immunol 2004, 282: 49–88.PubMedGoogle Scholar
- Jezyk MR, Snyder JT, Gershberg S, Worthylake DK, Harden TK, Sondek J: Crystal structure of Rac1 bound to its effector phospholipase C-beta2. Nat Struct Mol Biol 2006, 13(12):1135–1140. 10.1038/nsmb1175View ArticlePubMedGoogle Scholar
- Worthylake DK, Rossman KL, Sondek J: Crystal structure of the DH/PH fragment of Dbs without bound GTPase. Structure 2004, 12(6):1078–1086. 10.1016/j.str.2004.03.021View ArticlePubMedGoogle Scholar
- Lu M, Kinchen JM, Rossman KL, Grimsley C, deBakker C, Brugnera E, Tosello-Trampont AC, Haney LB, Klingele D, Sondek J, Hengartner MO, Ravichandran KS: PH domain of ELMO functions in trans to regulate Rac activation via Dock180. Nat Struct Mol Biol 2004, 11(8):756–762. 10.1038/nsmb800View ArticlePubMedGoogle Scholar
- Philip F, Guo Y, Scarlata S: Multiple roles of pleckstrin homology domains in phospholipase Cbeta function. FEBS Lett 2002, 531(1):28–32. 10.1016/S0014-5793(02)03411-7View ArticlePubMedGoogle Scholar
- Edlich C, Stier G, Simon B, Sattler M, Muhle-Goll C: Structure and phosphatidylinositol-(3,4)-bisphosphate binding of the C-terminal PH domain of human pleckstrin. Structure 2005, 13(2):277–286. 10.1016/j.str.2004.11.012View ArticlePubMedGoogle Scholar
- Zhang Y: Studies of the functions of pleckstrin in blood platelets : interactions of pleckstrin with phospholipids and soluble platelet proteins. In PhD Thesis. McMaster University, Department of Medical Sciences; 2005.Google Scholar
- Jackson SG, Zhang Y, Bao X, Zhang K, Summerfield R, Haslam RJ, Junop MS: Structure of the carboxy-terminal PH domain of pleckstrin at 2.1 Angstroms. Acta Crystallogr D Biol Crystallogr 2006, 62(Pt 3):324–330. 10.1107/S0907444905043179View ArticlePubMedGoogle Scholar
- Jackson SG, Zhang Y, Zhang K, Bao X, Schultz C, Haslam R, Junop M: Structural analysis of the binding of myo-inositol pentakisphosphates by the C-terminal PH domain of pleckstrin. FASEB J 2007, 21(5):A629-a.Google Scholar
- Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA: Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains. Mol Cell 2000, 6(2):373–384. 10.1016/S1097-2765(00)00037-XView ArticlePubMedGoogle Scholar
- Smith CI, Islam KB, Vorechovsky I, Olerup O, Wallin E, Rabbani H, Baskin B, Hammarstrom L: X-linked agammaglobulinemia and other immunoglobulin deficiencies. Immunol Rev 1994, 138: 159–183. 10.1111/j.1600-065X.1994.tb00851.xView ArticlePubMedGoogle Scholar
- Baraldi E, Carugo KD, Hyvonen M, Surdo PL, Riley AM, Potter BV, O'Brien R, Ladbury JE, Saraste M: Structure of the PH domain from Bruton's tyrosine kinase in complex with inositol 1,3,4,5-tetrakisphosphate. Structure 1999, 7(4):449–460. 10.1016/S0969-2126(99)80057-4View ArticlePubMedGoogle Scholar
- Berridge MJ: Inositol trisphosphate and calcium signalling. Nature 1993, 361(6410):315–325. 10.1038/361315a0View ArticlePubMedGoogle Scholar
- York JD, Guo S, Odom AR, Spiegelberg BD, Stolz LE: An expanded view of inositol signaling. Adv Enzyme Regul 2001, 41: 57–71. 10.1016/S0065-2571(00)00025-XView ArticlePubMedGoogle Scholar
- Irvine RF, Schell MJ: Back in the water: the return of the inositol phosphates. Nat Rev Mol Cell Biol 2001, 2(5):327–338. 10.1038/35073015View ArticlePubMedGoogle Scholar
- Mayr GW: A novel metal-dye detection system permits picomolar-range h.p.l.c. analysis of inositol polyphosphates from non-radioactively labelled cell or tissue specimens. Biochem J 1988, 254(2):585–591.PubMed CentralView ArticlePubMedGoogle Scholar
- Stephens LR, Hawkins PT, Stanley AF, Moore T, Poyner DR, Morris PJ, Hanley MR, Kay RR, Irvine RF: myo-inositol pentakisphosphates. Structure, biological occurrence and phosphorylation to myo-inositol hexakisphosphate. Biochem J 1991, 275(Pt 2):485–499.PubMed CentralView ArticlePubMedGoogle Scholar
- Shears SB: Metabolism of inositol phosphates. Adv Second Messenger Phosphoprotein Res 1992, 26: 63–92.PubMedGoogle Scholar
- McConnell FM, Stephens LR, Shears SB: Multiple isomers of inositol pentakisphosphate in Epstein-Barr-virus- transformed (T5–1) B-lymphocytes. Identification of inositol 1,3,4,5,6-pentakisphosphate, D-inositol 1,2,4,5,6-pentakisphosphate and L-inositol 1,2,4,5,6-pentakisphosphate. Biochem J 1991, 280(Pt 2):323–329.PubMed CentralView ArticlePubMedGoogle Scholar
- Shen X, Xiao H, Ranallo R, Wu WH, Wu C: Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates. Science 2003, 299(5603):112–114. 10.1126/science.1078068View ArticlePubMedGoogle Scholar
- Steger DJ, Haswell ES, Miller AL, Wente SR, O'Shea EK: Regulation of chromatin remodeling by inositol polyphosphates. Science 2003, 299(5603):114–116. 10.1126/science.1078062PubMed CentralView ArticlePubMedGoogle Scholar
- Zhou D, Chen LM, Hernandez L, Shears SB, Galan JE: A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Mol Microbiol 2001, 39(2):248–259. 10.1046/j.1365-2958.2001.02230.xView ArticlePubMedGoogle Scholar
- Deleu S, Choi K, Reece JM, Shears SB: Pathogenicity of Salmonella: SopE-mediated membrane ruffling is independent of inositol phosphate signals. FEBS Lett 2006, 580(7):1709–1715. 10.1016/j.febslet.2006.02.019PubMed CentralView ArticlePubMedGoogle Scholar
- Piccolo E, Vignati S, Maffucci T, Innominato PF, Riley AM, Potter BV, Pandolfi PP, Broggini M, Iacobelli S, Innocenti P, Falasca M: Inositol pentakisphosphate promotes apoptosis through the PI 3-K/Akt pathway. Oncogene 2004, 23(9):1754–1765. 10.1038/sj.onc.1207296View ArticlePubMedGoogle Scholar
- Maffucci T, Piccolo E, Cumashi A, Iezzi M, Riley AM, Saiardi A, Godage HY, Rossi C, Broggini M, Iacobelli S, Potter BV, Innocenti P, Falasca M: Inhibition of the phosphatidylinositol 3-kinase/Akt pathway by inositol pentakisphosphate results in antiangiogenic and antitumor effects. Cancer Res 2005, 65(18):8339–8349. 10.1158/0008-5472.CAN-05-0121View ArticlePubMedGoogle Scholar
- Luo HR, Huang YE, Chen JC, Saiardi A, Iijima M, Ye K, Huang Y, Nagata E, Devreotes P, Snyder SH: Inositol pyrophosphates mediate chemotaxis in Dictyostelium via pleckstrin homology domain-PtdIns(3,4,5)P3 interactions. Cell 2003, 114(5):559–572. 10.1016/S0092-8674(03)00640-8View ArticlePubMedGoogle Scholar
- Otwinowski Z, Minor W: Processing of X-ray Diffraction Data Collected in Oscillation Mode. Methods in Enzymology 1997, 276(Macromolecular Crystallography, part A):307–326.View ArticleGoogle Scholar
- Vagin A, Teplyakov A: MOLREP: an automated program for molecular replacement. J Appl Cryst 1997, 30: 1022–1025. 10.1107/S0021889897006766View ArticleGoogle Scholar
- Emsley P, Cowtan K: Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 2004, 60(Pt 12 Pt 1):2126–2132. 10.1107/S0907444904019158View ArticlePubMedGoogle Scholar
- Collaborative Computational Project, Number 4: The CCP4 suite : programs for protein crystallography. Acta Crystallogr D Biol Crystallogr 1994, 50(Pt 5):760–763. 10.1107/S0907444994003112View ArticleGoogle Scholar
- Murshudov GN, Vagin AA, Dodson EJ: Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 1997, 53(Pt 3):240–255. 10.1107/S0907444996012255View ArticlePubMedGoogle Scholar
- The PyMOL Molecular Graphics System[http://www.pymol.org]