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Table 3 Scaled up ZHX homeodomain purification yields and crystallization trials

From: Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

Protein Construct OPPF_ Yield/(mg/l) Number of 96 well plates Structure
ZHX1 1612 4.5 16  
  1613 2.1 10  
  1615 2.3 14  
  1632 1.8 12  
  1633 2.3 6  
  1635 3.5 17  
  1636 5.9 11  
  2264 4.1 12  
  2265 3.9 32 Yes
ZHX2 2268 11.5 25  
  2273 5.0 14 Yes
ZHX3 2274 2.5 8  
  2279 6.3 8  
  1. An additional construct containing the double ZHX1 zinc-finger domain was expressed & protein purified leading to a three-dimensional structure determined using NMR [19].