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Table 3 Scaled up ZHX homeodomain purification yields and crystallization trials

From: Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

Protein

Construct OPPF_

Yield/(mg/l)

Number of 96 well plates

Structure

ZHX1

1612

4.5

16

 
 

1613

2.1

10

 
 

1615

2.3

14

 
 

1632

1.8

12

 
 

1633

2.3

6

 
 

1635

3.5

17

 
 

1636

5.9

11

 
 

2264

4.1

12

 
 

2265

3.9

32

Yes

ZHX2

2268

11.5

25

 
 

2273

5.0

14

Yes

ZHX3

2274

2.5

8

 
 

2279

6.3

8

 
  1. An additional construct containing the double ZHX1 zinc-finger domain was expressed & protein purified leading to a three-dimensional structure determined using NMR [19].