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Table 1 RACK1 hydrodynamic properties.

From: Solution structure of the human signaling protein RACK1

RACK1 Hydrodynamic property

Predicted for a sphere

DLS*

AUC

HydroPro of homology model

SAXS

M (kDa)

37.2

36.4 ± 0.8&

36.4 ± 0.8&

-

40

s020,w (S)

4.01

-

3.00 ± 0.01

3.16 ± 0.02

-

D20,w (10-7cm2/seg)

9.6

7.7 ± 0.2

-

8.0 ± 0.1

-

Dmax (Å)

-

-

-

63 ± 1

60

Rg (Å)

-

-

-

20.0 ± 0.1

20.8 ± 0.8$

     

20.6 ± 0.4#

  1. & from s/D ratio (Equation 1)
  2. $: from Guinier approximation
  3. #: from the normalized second moment of the p(r) function
  4. * Dynamic light scattering