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Table 1 NcdT436S kinetics and motility

From: A kinesin motor in a force-producing conformation

Motor ADP Release*
koff (s-1)
ATPase Velocity
(μm/min)
  No MTs
+ ATP
+ MTs + MTs, ATP No MTs
k cat (s -1 )
+ MTs
k cat (s -1 )
K m,MTs
(μM)
Leading
end
Lagging
end
NcdT436S 0.0304 ± 0.0005
(n = 6)
0.014 ± 0.003
(n = 4)
0.066 ± 0.009
(n = 3)
0.28 ± 0.10
(n = 4)
2.2 ± 0.4
(n = 4)
1.3 ± 0.6
(n = 4)
6.8 ± 0.1
(n = 22)
7.6 ± 0.2
(n = 22)
WT Ncd 0.00305 ± 0.00002
(n = 6)
0.018 ± 0.006
(n = 6)
0.04 ± 0.01
(n = 6)
0.10 ± 0.04
(n = 4)
1.0 ± 0.2
(n = 4)
1.6 ± 0.8
(n = 4)
10.2 ± 0.7
(n = 32)
10.9 ± 0.7
(n = 31)
  1. MTs, microtubules. Mean ± sem.
  2. *No MTs assays in HEM100; + MTs assays in HEM50