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Table 1 NcdT436S kinetics and motility

From: A kinesin motor in a force-producing conformation

Motor

ADP Release*

koff (s-1)

ATPase

Velocity

(μm/min)

 

No MTs

+ ATP

+ MTs

+ MTs, ATP

No MTs

k cat (s -1 )

+ MTs

k cat (s -1 )

K m,MTs

(μM)

Leading

end

Lagging

end

NcdT436S

0.0304 ± 0.0005

(n = 6)

0.014 ± 0.003

(n = 4)

0.066 ± 0.009

(n = 3)

0.28 ± 0.10

(n = 4)

2.2 ± 0.4

(n = 4)

1.3 ± 0.6

(n = 4)

6.8 ± 0.1

(n = 22)

7.6 ± 0.2

(n = 22)

WT Ncd

0.00305 ± 0.00002

(n = 6)

0.018 ± 0.006

(n = 6)

0.04 ± 0.01

(n = 6)

0.10 ± 0.04

(n = 4)

1.0 ± 0.2

(n = 4)

1.6 ± 0.8

(n = 4)

10.2 ± 0.7

(n = 32)

10.9 ± 0.7

(n = 31)

  1. MTs, microtubules. Mean ± sem.
  2. *No MTs assays in HEM100; + MTs assays in HEM50