Figure 2

Oligomerization state analysis of sGC CC domain. Size exclusion gel filtration chromatography of the CC sGCβ1 using a Superdex 75 column resulted in an oligomeric 38.5 kD species (thick arrow) and a minor 19.5 kD species (thin arrow). The column was calibrated using a mixture of different Mw standards (interrupted line). Inset, Native polyacrylamide gel electrophoresis analysis of CC sGCβ1 (lane 1) and a mixture of 3 Mw standards (lane M) revealing a major and minor species with similar Mw as obtained by size-exclusion chromatography (thick and thin arrow, respectively).