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Figure 4 | BMC Structural Biology

Figure 4

From: Structural motif screening reveals a novel, conserved carbohydrate-binding surface in the pathogenesis-related protein PR-5d

Figure 4

A predicted binding-site in PR-5d and experimental validation of PR-5d insoluble-carbohydrate binding activity using a cellulose pulldown assay and mass spectrometry. (A) The predicted Trp-rich binding surface in tobacco antifungal protein, PR-5d (PDB ID 1aun). A surface representation is also shown to illustrate the hydrolytic cleft region, which is distinct from the predicted binding site. (B) A silver stained gel of purified cellulose-binding proteins from tobacco (left). The marked band was excised from the corresponding position on a coomassie stained gel and identified by mass spectrometry (MS). MS sequencing identified two peptides matching the PR-5d sequence. MS statistics and sequence information is shown to the right of the gel, where CamC represents carbamidomethyl derivatives of cysteine residues.

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