Skip to main content
Figure 4 | BMC Structural Biology

Figure 4

From: Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2ΔGF122) and structural roles of F122 in target recognition

Figure 4

Effects of amino acid substitutions of F122 on binding specificities. ALG-2-interacting proteins in HEK293 cells were pulled down with GST-fused ALG-2 of wild type (WT), ALG-2ΔGF122 (ΔGF122), and F122-substituted mutants. Unfused GST was used as a negative control (Ctrl). Proteins bound to glutathione Sepharose beads were subjected to Western blotting with antibodies against Alix, TSG101, Sec31A, annexin A7, and annexin A11.

Back to article page

BMC Structural Biology

ISSN: 1472-6807

Contact us