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Figure 1 | BMC Structural Biology

Figure 1

From: Molecular determinants of improved cathepsin B inhibition by new cystatins obtained by DNA shuffling

Figure 1

Expression and purification of phytocystatins and mutants. SDS-PAGE 15% stained with Coomassie blue showing OC-I, CaneCPI-1, CaneCPI-4, OC-I NΔ, A10, mutant 1, mutant 2 and mutant 3. Samples were collected and analyzed in SDS-PAGE 15%. In (1) molecular mass marker; (2) E. coli Rosetta (DE3) cell extract before and (3) after IPTG induction, (4) insoluble and (5) soluble fractions after disruption of induced cells from E. coli Rosetta (DE3), and (6) purified recombinant protein after elution with 250 mM imidazole from a nickel column.

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