Figure 2
Presence of cis and trans proline isomers in N-terminal s Vpr. A 1 : Fingerprint region of the 2D 1H-1H TOCSY spectrum showing the assigned spin systems of residues close to proline residues in s Vpr(1-20) and A 2 : Superimposed expanded HN-HN regions of the 2D 1H-1H NOESY spectra of s Vpr1-20 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 50 μl (0.1 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA. B 1 : Expanded Hα-Hβ region of the 2D 1H-1H TOCSY spectrum of s Vpr21-40 in H2O:D2O (9:1, v/v) at pH 7 showing the signals of trans and cis Pro-35. B 2 : Superimposed expanded Hα-Hβ regions of the 2D 1H-1H NOESY spectra of s Vpr21-40 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 100 μl (0.2 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA.