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Figure 4 | BMC Structural Biology

Figure 4

From: The intriguing Cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding

Figure 4

Catalysis of isomerisation of Pro-35 of Vpr21-40 by CypA. A, C, E: Superimposed expanded Hα-Hβ regions of the 2D 1H-1H NOESY spectra of s Vpr21-40 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 100 μl (0.2 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA. B, D, F: Superimposed expanded Hα-Hβ region of the 2D 1H-1H NOESY spectra of s Vpr21-40 in H2O:D2O (9:1, v/v) at pH 7 after addition of 100 μl (0.2 mg) CypA (green signals) and after additional addition of 5 μl (0.1 mg) cyclosporine A (red signals). Notice that the prolyl cis/trans related exchange peaks detected after addition of CypA disappear after addition of cyclosporine A.

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