Figures 5
Catalysis of isomerisation of Pro-5,10 and 14 of Vpr1-20 by CypA. A: Superimposed expanded HN-HN regions of the 2D 1H-1H NOESY spectra of s Vpr1-20 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 50 μl (0.1 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA. B: Superimposed expanded HN-HN regions of the 2D 1H-1H NOESY spectra of s Vpr1-20 in H2O:D2O (9:1, v/v) at pH 7 after addition of 50 μl (0.1 mg) CypA (green signals) and after additional addition of 5 μl (0.1 mg) cyclosporine A (red signals). Notice that the prolyl cis/trans related exchange peaks detected after addition of CypA disappear after addition of cyclosporine A.