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Figure 8 | BMC Structural Biology

Figure 8

From: The intriguing Cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding

Figure 8

Characterization of CypA binding region of the N-terminus of Vpr. Synthetic Vpr30-40 (A), Vpr32-38 (B), Vpr33-37(C, D) were tested for binding to immobilized recombinant CypA using SPR biosensor system. Individual peptides were injected at concentration ranging from 0-400 μM over CM5 chip immobilized with 980 RU CypA. The curves were best fit to a two state model, and ka1, ka2, kd1, kd2 and KD were calculated for the respective sensograms (Table 1.).

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