Table 2 Recombinant hNek6 Stokes radii (Rs) determined by analytical size-exclusion chromatography.
From: Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain
Protein | Rs(nm) | Ve(mL)a | Apparent Rs(nm)a |
|---|---|---|---|
Conalbumin | 3.6 | 14.9 ± 0.1 | 3.5 ± 0.2 |
Ovalbumin | 3.0 | 15.8 ± 0.1 | 2.9 ± 0.0 |
Carbonic Anhydrase | 2.0 | 16.9 ± 0.1 | 2.2 ± 0.0 |
Ribonuclease | 1.6 | 18.2 ± 0.0 | 1.4 ± 0.1 |
Nek6wt |  | 16.4 ± 0.3 | 2.6 ± 0.1 |
Nek6wtD |  | 17.1 ± 0.0 | 2.1 ± 0.0 |
Nek6(S206A) |  | 16.7 ± 0.2 | 2.4 ± 0.0 |
Nek6(S206A)D |  | 17.1 ± 0.0 | 2.1 ± 0.0 |
Nek6(Δ1-44) |  | 17.6 ± 0.3 | 1.8 ± 0.1 |