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Table 3 Recombinant hNek6 weight average molecular masses (Mw) determined by SEC-MALS and melting temperatures (Tm) during thermal shift denaturation.

From: Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain

Protein

Pred. M(kDa)a

Mw(kDa)b

Apparent Tm(°C)c

Nek6wt

37.7

38.4 ± 0.6

39.5 ± 0.1

Nek6wtD

37.7

37.8 ± 2.2

40.8 ± 0.1

Nek6(S206A)

37.7

38.1 ± 2.1

38.0 ± 0.1

Nek6(S206A)D

37.7

38.4 ± 3.3

36.8 ± 0.3

Nek6(Δ1-44)

33.2

33.3 ± 1.6

41.0 ± 0.2

  1. a Molecular masses predicted by ProtParam tool [60].
  2. b S.E. of two measurements at different buffers.
  3. c S.E. of three measurements.