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Figure 3 | BMC Structural Biology

Figure 3

From: Structural studies of the mechanism for biosensing antibiotics in a fluorescein-labeled β-lactamase

Figure 3

Crystal structure of PenP-E166Cf-cefotaxime. (a) The fo-fc map of cefotaxime in PenP-E166Cf-cefotaxime complex contoured at 2.0 σ. The light blue dash line represents the disordered Arg164 to Pro174 due to the poor electron density. (b) Comparison of PenP-E166Cf-cefotaxime complex with apo PenP-E166Cf structure. The two structures are superimposed by main chain atoms. Key residues including Cys166, Ser70 and cefotaxime are also shown in cpk cylinder model. (c) Comparison of binding mode of cefotaxime in PenP-E166Cf with that of Toho-1 and GC-1. PenP-E166Cf, Toho-1 and GC1 are superimposed by aligning on overall main chain atoms. Cefotaxime is in cylinder model colored in cpk (PenP-E166Cf-cefotaxime), golden (Toho-1) and red (GC1) respectively.

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