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Table 2 TlyA subcellular localization prediction through 9 available servers for prokaryotic proteins

From: Molecular modeling and in silico characterization of Mycobacterium tuberculosis TlyA: Possible misannotation of this tubercle bacilli-hemolysin

Tool

Subcellular localization

Value

Reliability Index

Method

Overall prediction accuracy

Ref.

TBpred

Cytoplasmic

1.365

Higher value above 0 or 1

SVM/PSSM, MEME/MAST, HM.

82.51, 80.39 and 86.62%

24

Gpos-PLoc

Cytoplasmic

ND

ND

By fusing PseAAC

> 80%

25

PSORTb

Cytoplasmic

8.870

≥ 7.5

SCL-BLAST, SVM, Motif and Profile Analysis

96%

26

CELLO

Cytoplasmic

3.809

Higher value

SVM based on multiple n-peptide composition

89%

27

LOCtree

Cytoplasmic

8.000

>3

SVM

ND

28

SubLoc v1.0

Cytoplasmic

5.000

5

SVM

96%

29

PA-SUB

Extracellular

72.84%

Higher percent

SVM

> 92%

30

MemType-2L

Non membrane protein

ND

ND

Pse-PSSM

92.7

31

TMBETA-SVM

Non membrane protein

0.636

ND

SVM

92%

32

  1. ND: non defined value. Pse-PSSM: Pseudo Position-Specific Score Matrix. SVM: support vector machines. PSSM: Position-Specific Scoring Matrix. MEME/MAST: Multiple Em for Motif Elicitation/Motif Alignment and Search Tool. HM: Hybrid module. PseAAC: Pseudo Amino Acid Composition.