Figure 5
Identification of the β3 region as the TPR1-interacting site of Gα 16 . (A) Schematic representation of the C186, N188, C174, and N200 chimeras. (B-C) HEK 293 cells were transiently co-transfected with FLAG-TPR1 and the wild-type or constitutively active mutants of Gα16, C186, N188, C174, N200, or pcDNA1. Co-immunoprecipitation assays were performed and analyzed as in Figure 2. Data shown represent one of three sets of immunoblots; two other sets yielded similar results.