Figure 12

Strictly conserved residues among all nine homologous sequences in table 2. a) view looking into the active site of the TIM barrel domain, with the C-terminal domain removed for clarity. The surface is shown in space filling form with main chain peptides and strictly conserved residues colored brick red, and side chains of non-conserved residues colored according to atom type with carbon atoms green. The Cα atoms of nonconserved residues are colored green stippled with red. Acetyl-CoA and pyruvate are depicted in stick form showing their binding locations in the ternary complex with carbon atoms colored slate blue or orange respectively. b) same as in part a, but showing C-terminal domain interactions.