Schematic representation of the domain structures of TraB. (A) N: N-terminus; C: C-terminus; TM: Putative transmembrane domain; NBD: NTP binding domain; FL: TraB full-length (TraBFL: 1-866); NT: TraB N-terminal domain (TraBNT: 1-442); CT: TraB C-terminal domain (TraBCT: 448-848). (B) NuPAGE 4-12% showing the purified proteins after gel filtration. (Lane 1) TraBFL (102 kDa); (Lane 2) TraBNT (55 kDa); (Lane 3) TraBCT (48.8 kDa); and (Lane 4) LvhB4 (93.8 kDa). Molecular mass markers are indicated on the left side of the gel (kDa). A transmembrane segment (TM) in TraB is predicted, between residues 254 and 271.