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Table 1 Theoretical and experimental Molecular Mass (MM) determination

From: Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system

  MMCalc GF calibration D.L.S BN-PAGE Oligomeric state
TraB FL 103 198 184 146 - 242 Dimer
TraB NT 55 122 ND ND Dimer
TraB CT 49 100 100 66 - 146 Dimer
LvhB4 97 93 105 ND Monomer
  1. All MM are given in kDa. "MMCalc" is the MM calculated from the amino-acid sequence, "GF" stands for gel filtration, "DLS" stands for Dynamic Light Scattering, "BN-PAGE" stands for Blue Native-PAGE. The oligomeric state is deduced from the comparison between MMCalc and the experimental MM as determined by the other approaches. "ND" stands for non-determined.