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Table 2 Biophysical parameters estimated by SAXS

From: Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system

Protein

MMCalc (kDa)

MMExp (kDa)

O.S

RG*(Ã…)

RG **(Ã…)

Dmax (Ã…)

RG**/Dmax

V(Ã…3)

GASBOR ChiExp

TraBFL

103

219

2

58.6 ± 0.6

58.8

198 ± 2

0.30

2.4 × 105

1.96

TraBNT

55

106

2

60.3 ± 0.7

59.2

195 ± 5

0.30

1.3 × 105

1.56

TraBCT

49

83

2

41.5 ± 0.5

46.1

150 ± 5

0.31

1.1 × 105

0.61

LvhB4

97

116

1

37.2 ± 0.6

38.1

120 ± 5

0.32

1.1 × 105

2.01

(TraBNT+TraBCT)/TraBFL

1.01

0.86

     

1.0

 

2(LvhB4)/TraBFL

0.94

1.06

     

0.92

 
  1. MMCalc: Theoretical molecular mass calculated from amino acid sequence. MMExp: Experimentally based molecular mass calculated from the scattering intensity extrapolated at zero angles I(0). O.S: Oligomeric state, as determined by comparison between the MMCalc and the MMExp. RG*: Radius of gyration, estimated from the Guinier plots. RG**: Radius of gyration, estimated using the program GNOM. V: volume of the envelope calculated by ab initio modelling. ChiExp: discrepancy between the experimental SAXS profile and the fit for each model calculated by the program GASBOR. The ratios of the Molecular Masses and of the volumes between different constructs are given in the last two lines.