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Table 2 Biophysical parameters estimated by SAXS

From: Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system

Protein MMCalc (kDa) MMExp (kDa) O.S RG*(Å) RG **(Å) Dmax (Å) RG**/Dmax V3) GASBOR ChiExp
TraBFL 103 219 2 58.6 ± 0.6 58.8 198 ± 2 0.30 2.4 × 105 1.96
TraBNT 55 106 2 60.3 ± 0.7 59.2 195 ± 5 0.30 1.3 × 105 1.56
TraBCT 49 83 2 41.5 ± 0.5 46.1 150 ± 5 0.31 1.1 × 105 0.61
LvhB4 97 116 1 37.2 ± 0.6 38.1 120 ± 5 0.32 1.1 × 105 2.01
(TraBNT+TraBCT)/TraBFL 1.01 0.86       1.0  
2(LvhB4)/TraBFL 0.94 1.06       0.92  
  1. MMCalc: Theoretical molecular mass calculated from amino acid sequence. MMExp: Experimentally based molecular mass calculated from the scattering intensity extrapolated at zero angles I(0). O.S: Oligomeric state, as determined by comparison between the MMCalc and the MMExp. RG*: Radius of gyration, estimated from the Guinier plots. RG**: Radius of gyration, estimated using the program GNOM. V: volume of the envelope calculated by ab initio modelling. ChiExp: discrepancy between the experimental SAXS profile and the fit for each model calculated by the program GASBOR. The ratios of the Molecular Masses and of the volumes between different constructs are given in the last two lines.