Figure 2

Results from protein expression: (A) SDS-PAGE 12% showing that the recombinant mutant proteins were > 95% pure. Molecular masses for standard proteins are shown on left. (B) CD spectra of recombinant proteins (10-40 μM) were measured from 195 to 260 nm in 25 mM Tris-HCl (pH 7.5) containing 500 mM NaCl and are shown as molar residual ellipticity ([Θ]).