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Table 2 Overall parameters derived from the SAXS results for the proteins Sis1, Sis1_Δ124-174, Sis1_Δ121-257 and Ydj1_Δ106-255

From: Central domain deletions affect the SAXS solution structure and function of Yeast Hsp40 proteins Sis1 and Ydj1

 

Protein

Structural properties

Sis1

Sis1_Δ 124-174

Sis1_Δ 121-257

Ydj1_Δ 106-255

C (mg/mL)

7.1, 3.1

6.8, 4.2

5.2, 3.9

5.5, 2.75

Rg (Ã…) (Guinier)

42 ± 1

42 ± 1

35 ± 1

33 ± 1

Rg (Ã…) (from p(r))

43.3 ± 0.5

42.6 ± 0.3

35.2 ± 0.3

34.2 ± 0.3

Dmax (Ã…)

160

140

125

110

M (kDa) (SAXS)

~71

~69

~42

~47

Predicted M (kDa)*

75.2

66.1

46.8

56.1

Oligomerization state

Dimer

Dimer

Dimer

Dimer

  1. * M predicted from amino acids sequence for all proteins as dimers.