Skip to main content

Table 2 Overall parameters derived from the SAXS results for the proteins Sis1, Sis1_Δ124-174, Sis1_Δ121-257 and Ydj1_Δ106-255

From: Central domain deletions affect the SAXS solution structure and function of Yeast Hsp40 proteins Sis1 and Ydj1

  Protein
Structural properties Sis1 Sis1_Δ 124-174 Sis1_Δ 121-257 Ydj1_Δ 106-255
C (mg/mL) 7.1, 3.1 6.8, 4.2 5.2, 3.9 5.5, 2.75
Rg (Å) (Guinier) 42 ± 1 42 ± 1 35 ± 1 33 ± 1
Rg (Å) (from p(r)) 43.3 ± 0.5 42.6 ± 0.3 35.2 ± 0.3 34.2 ± 0.3
Dmax (Å) 160 140 125 110
M (kDa) (SAXS) ~71 ~69 ~42 ~47
Predicted M (kDa)* 75.2 66.1 46.8 56.1
Oligomerization state Dimer Dimer Dimer Dimer
  1. * M predicted from amino acids sequence for all proteins as dimers.