Detailed view of hydration at the protein/DNA interface. The Sso7d binding region (grey) with a DNA duplex (green) is shown. Water molecules arranged in a diamond shaped cluster, always found at the Sso7d/Sac7d-DNA binding interface, are colored in red; other water molecules that were found to be conserved in the available crystal structures are shown as purple spheres. The heavy atoms of the amino acid side chains in the presence of ePHOGSY signals are in cyan. The MDHS are shown in yellow; the mesh iso-surfaces were at the 2.8 level.