Table 1 MIC values of Moxifloxacin, gatifloxacin and ofloxacin on various strains of Mycobacterium tuberculosis comprising the four mutant codons in QRDR region of gyrA.
Strain data | S. No. | Strain No. | MDR status | Resistance to Ofloxacin | Area | MFX (μg/ml) | GFX (μg/ml) | OFX (μg/ml) |
|---|---|---|---|---|---|---|---|---|
First mutant - Codon 90 Ala-Val | 1 | 944284 | MDR | R(M) | Goa | 5 | 5 | 8 |
| Â | 2 | 940228 | MDR | R(L) | Chennai | NA | NA | 32 |
Binding affinity (kcal/mol) | -7.9 | -7.3 | -7.4 | |||||
Second mutant - Codon 94 Asp-Asn | 3 | 936837 | MDR | R(H) | Sikkim | NA | NA | 64 |
Binding affinity (kcal/mol) | -7.9 | -7.3 | -7.4 | |||||
Third mutant - Codon 94 Asp-Ala | 4 | 912607 | MDR | R(H) | Chennai | NA | NA | 64 |
| Â | 5 | 938424 | MDR | R(H) | Karnataka | 5 | 5 | 16 |
| Â | 6 | 937532 | MDR | R(M) | Chennai | 2 | 5 | 8 |
| Â | 7 | 939784 | MDR | R(M) | Chennai | > 5 | 2 | 32 |
| Â | 8 | 940183 | MDR | R(L) | Sikkim | NA | NA | 16 |
| Â | 9 | RF0048 | MDR | R(H) | NA | 5 | 5 | 16 |
Binding affinity (kcal/mol) | -7.9 | -7.4 | -7.1 | |||||
Fourth mutant - Codon 95 Ser-Thr (Natural polymorphism) | 10 | 910659 | MDR | S | Chennai | 0.25 | 1 | 2 |
| Â | 11 | 913168 | MDR | R(L) | Chennai | NA | 8 | 16 |
| Â | 12 | 916590 | MDR | R(M) | Tamil Nadu | NA | 8 | 32 |
| Â | 13 | 929925 | MDR | R(L) | Chennai | 1 | 1 | 8 |
| Â | 14 | 932503 | MDR | S | Chennai | 0.5 | 0.5 | 2 |
| Â | 15 | 933750 | MDR | R(H) | NA | 5 | 5 | 8 |
| Â | 16 | 932504 | MDR | R(L) | Chennai | 2 | 2 | 8 |
| Â | 17 | 937583 | MDR | R(L) | Chennai | 1 | 2 | 16 |
| Â | 18 | 937911 | MDR | R(H) | UP | > 5 | > 5 | 32 |
| Â | 19 | 938066 | Non MDR | S | Chennai | NA | NA | 4 |
| Â | 20 | 939184 | MDR | R(L) | Chennai | NA | NA | 8 |
| Â | 21 | 939271 | MDR | S | Chennai | NA | NA | 4 |
| Â | 22 | 940586 | MDR | R(H) | AP | 5 | > 5 | 64 |
| Â | 23 | 940742 | Non MDR | S | Chennai | 1 | 0.5 | 4 |
| Â | 24 | 941935 | INH resistant RMP sensitive | R(L) | Chennai | NA | NA | 8 |
| Â | 25 | 942100 | MDR | R(L) | Chennai | 5 | 5 | 16 |
| Â | 26 | 947356 | MDR | R(M) | Chennai | 5 | 2 | 32 |
| Â | 27 | RF0071 | MDR | R(L) | NA | NA | NA | 8 |
| Â | 28 | 939825 | MDR | S | Chennai | 1 | 1 | 4 |
| Â | 29 | 939850 | Non MDR | S | Chennai | 0.25 | 0.5 | 2 |
| Â | 30 | 940031 | MDR | S | Chennai | 0.25 | 0.5 | 4 |
| Â | 31 | 940033 | INH resistant RMP Sensitive | S | Chennai | 0.5 | 0.5 | 2 |
| Â | 32 | RF0057 | Non MDR | S | NA | NA | NA | 2 |
| Â | 33 | RF0095 | MDR | S | NA | 0.5 | 0.2 | 2 |
| Â | 34 | RF0096 | MDR | S | NA | 0.125 | 0.2 | 2 |
| Â | 35 | RF0119 | MDR | S | NA | NA | NA | 2 |
| Â | 36 | RF0130 | MDR | S | NA | 0.25 | 0.2 | 2 |
| Â | 37 | RF0131 | MDR | S | NA | 0.25 | 0.5 | 2 |
| Â | 38 | 949998 | Non MDR | S | Chennai | 1 | 0.5 | 4 |
| Â | 39 | 950629 | MDR | S | Chennai | 1 | 0.5 | 2 |
Binding affinity (kcal/mol) | -7.9 | -7.3 | -7.6 | |||||
Wild type (H37 Rv) | Â | Â | Â | Â | Â | 0.25 | 0.25 | < 2 |
Binding affinity (kcal/mol) | -7.9 | -7.4 | -7.6 | |||||