Figure 2

Pv Trx-Px1 and Py Trx-Px1 active sites. (A) Active sites of Pv Trx-Px1_red and Pv Trx-Px1_ox are shown in red/dark grey and orange/light grey, respectively. Note the positioning of Pro43, Thr47, and Arg125 are unchanged between the reduced and oxidized forms. The dramatic change of the active site C_P in the reduced form (red) is shown as untwisting of the helix to meet the C_R from its dimeric partner. As well, the formation of the disulfide results in the disruption of the final C-terminal α-helix. Note that in the reduced form the C_P and C_R are separated by 13.5 Å. (B) Active site of Py Trx-Px1_ox is shown for comparison.