Figure 6

Role of C -terminal tail and NNLA(I/L)GRS-loop in the determination the oligomerization of Py Prx-Tr1. (A) and (B) Two views of the oxidized monomers of Py Trx-Px1 (blue) and Pv Trx-Px1 (yellow) showing their structural differences: C-terminal tail adopts a different orientation in each structure, NNLA(I/L)GRS-loop also adopts a different orientation in each structure (see panel B), and the Arg adopts a surface position in Py Trx-Px1 and a buried position in Pv Trx-Px1. Note that chain I from PDB: 2H66 is used because it is most complete at the C-terminus. As well, portions of the structures in (B) are hidden to facilitate the view of the loop (including up to Tyr42 from the N-terminus and from Ala56 to Asp 77). (C) In order to demonstrate how the C- terminal tail positions itself in the dimer, a hybrid surface-cartoon rendering of Py Trx-Px1 shows the orientation of the C-terminal tail and the NNLA(I/L)GRS loop in grey. (D) and (E) For comparison, the hybrid surface-cartoon renderings of Pv Trx-Px1 show the orientation of the C-terminal tail and the NNLA(I/L)GRS loop in grey. Note that (D) is from chains I and J which only shows a complete C-terminal chain for I. As well, for (E) chains C and D are used which both truncate around C_R for the C-terminal tail, but show that the C-terminus is the same up to this point.