Table 1 Representative Tyk2 expression constructs
Rationale | Constructs |
|---|---|
Orthologs | Human (hu) |
Mouse (mu) | |
Variation of affinity tag | His-Tev-huTyk2 (891–1185) |
His-Tev-huTyk2 (880–1185)-Flag | |
GST-Thrombin-huTyk2 (870–1180) | |
GST-Tev-muTyk2 (870-1170) | |
N- and C-terminal variations | huTyk2 (880–1185) huTyk2 (891–1185) |
huTyk2 (891–1175) | |
huTyk2 (589–1185) | |
Kinase-inactive mutations | huTyk2 (870–1180) D1023A |
muTyk2 (870-1170) D1016A |