Figure 5
A. Carbohydrates in the N9-compound 2 model at 1.55 Å resolution. Carbon atoms are green for N9 NA and the N-linked glycans attached at positions 86, 146 and 200 that are in common with other structures. Mannose residues not previously seen in N9 structures (blue) and the four glucose molecules from the cryoprotectant (yellow) are shown. Compound 2 is colored gray. B. Electron density (2FoFc at 1σ) for the glycan at Asn200. Also shown is the cartoon of the glycan structure using standard Consortium for Functional Glycomics symbols (green circle, mannose; blue square, N-acetylglucosamine) made with Glycan Builder [45]. C. Interaction of the glycan attached to Asn200 (green) with the neighboring subunit (gray) built by applying crystallographic symmetry.