Figure 11From: Crosslinking and mass spectrometry suggest that the isolated NTD domain dimer of Moloney murine leukemia virus integrase adopts a parallel arrangement in solution Cysteine cross-linking. The reaction was carried out in 50 mM Tris–HCl pH 7.0. 50 μM of IN 1–105 K104C in buffer without crosslinker (control lane) or in the presence of 1 mM of the bi-functional maleimide crosslinker BMOE or BM(PEG)2. The reaction was done on ice for 60 min and quenched with 10 mM DTT.Back to article page