Figure 9
Epitope-mapping results. A. MS spectra of the proteolytic-digestion products of c5321 with GluC (upper spectrum) and the peptide immunocaptured with mAb14E7/D10 (111-SVKWFRLAAEQGRDSGQQSMGDAYFE-136, lower spectrum). B. MS spectra of the proteolytic-digestion products of c5321 with LysC (upper spectrum) and the peptide immunocaptured with mAb16H8/G6 (18-AQLELGYRYFQGNETTK-34, lower spectrum). C. MS spectra of the proteolytic-digestion products of c5321 with GluC (upper spectrum) and the peptide immunocaptured with mAb17A7-C2 (340-KAAQFNLGNALLQG-KGVKKDE-360, lower panel). D. Identified epitope-containing regions mapped onto the c5321 structure.