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Table 1 Kinetic parameters of MATs from different domains of life

From: Structural and functional characterisation of the methionine adenosyltransferase from Thermococcus kodakarensis

  Tk MAT Mj MAT Ec MAT Hs MAT
kinetic parameters     
V max [μmol/min/mg] 1.95 ± 0.26 3.0 1.2 0.2
K M (Met) [mM] 0.31 ± 0.07 0.14 0.08 0.003
K M (ATP) [mM] 6.54 ± 2.49 0.25 0.11 0.03
assay HPLC (SCX column) radioactive filter binding assay radioactive filter binding assay radioactive filter binding assay
buffer 0.1 M Tris–HCl, 0.02 M MgCl2, 0.2 KCl, pH 8.0 50 mM K-Hepes, 10 mM MgCl2, 25 mM KCl, pH 8.0 0.1 Tris-Cl, 6 mM MgCl2, 0.1 KCl, pH 8.3 50 mM K-Tes, 50 mM KCl, 10 mM MgCl2, 10 mM DTT, 0.3 mM Na2EDTA, 0.1 mM BSA, pH 7.4
substrates 10 mM l-methionine 10 mM ATP 0.1 mM l-[methyl-14C]-methionine 5 mM ATP 0.6 mM l-[methyl-14C]-methionine 4.4 mM 14C-ATP 0.01 mM l-[methyl-14C]-methionine 5 mM ATP
temperature 37°C 70°C 25°C 37°C
reference this work Graham et al. [16] Markham et al. [12] Kotb & Kredich [25]