Table 2 Primers used for site-directed mutagenesis to produce Cys to Ser mutants

C89S

For: 5’ CTTCTACGAGGATTTCCTGTCTCCGGCGTGCGGCATATTC 3’

Rev: 5’ GAATATGCCGCACGCCGGAGACAGGAAATCCTCGTAGAAG 3’

C92S

For: 5’ GAGGATTTCCTGTGTCCGGCGTCCGGCATATTCGAGCGCGGTTCGG 3’

Rev: 5’ CCGAACCGCGCTCGAATATGCAGGCCGCCGGACACAGGAAATCCTC 3’

C140S

For: 5’ CTGCTGCGGCTTATTCCGTTGCCGACGAATC 3’

Rev: 5’ GATTCGTCGGCAACGGAATAAGCCGCAGCAG 3’

C192S

For: 5’ CAAGGTGCCCGACTCCATCAACAGCGGCAAG 3’

Rev: 5’ CTTGCCGCTGTTGATGGAGTCGGGCACCTTG 3’