Structure of the ABCC11 complex for molecular dynamics simulation and RMSD calculations. (A) The inward facing model of ABCC11 was inserted in a phospholipid bilayer membrane surrounded by solvent. (B) 1D-RMSD of the ABCC11 complex during the dynamics simulation before (grey) and after (black) reaching equilibrium state. (C) 2D-RMSD calculations (X and Y axes represent the frames), carried out on 500 conformations selected with a stride of 2 from the 1000 produced during the indicated time simulation. The four steady-state conformations of each simulation are numbered. Warm colors (yellow and white) revealed steady state was reached.