Figure 6
From: A structural role for the PHP domain in E. coli DNA polymerase III
Mutations at the PHP domain decrease the overall stability of E. coli Pol III. The thermal and chemical stability of Pol III decreases gradually with the number of mutations introduced at the PHP domain, as measured by (A) temperature melt followed by circular dichroism or (B) through chemical denaturation using guanidine-hydrochloride titrations followed by circular dichroism and tryptophan fluorescence. Pol III shows apparent two-state unfolding.