BMC Structural Biology

Table 1 Conservation of the nine residues required for metal binding in PHP domains

From: A structural role for the PHP domain in E. coli DNA polymerase III

		Metal binding residue									Metal
	PDB	1	2	3	4	5	6	7	8	9	Me1	Me2	Me3
Consensus		H	H	D/H	H	E	H	C/H	D/N	H	Zn
Eco Pol III	2hnh	R₁₀	H₁₂	D₁₉	F₄₄	D₆₉	H₈₃	G₁₃₄	D₂₀₁	R₂₀₃	R₂₀₃	R₁₀	-
3mPHP	4jom	H ₁₀	"	"	H ₄₄	"	"	"	"	H ₂₀₃	Zn	Zn	-
4mPHP	n.a.	H ₁₀	"	"	H ₄₄	"	"	C ₁₃₄	"	H ₂₀₃	n.a.	n.a.	n.a.
5mPHP	n.a.	H ₁₀	"	"	H ₄₄	E ₆₉	"	C ₁₃₄	"	H ₂₀₃	n.a.	n.a.	n.a.
Taq Pol III	2hpi	H₁₁	H₁₃	D₂₀	H₄₇	E₇₂	H₉₅	C₁₄₅	D₂₁₂	H₂₁₄	Zn	Zn	-
Gka PolC	3f2d	H₃₄₆	H₃₄₈	D₃₅₅	H₃₈₀	E₄₀₅	H₆₂₀	C₆₇₀	N₇₄₃	H₇₄₅	Zn	Mn	Mn

Mutations introduced in the PHP domain of Eco Pol III are indicated in bold. M1, M2, M3 are metal positions going from outside to inside of the PHP ligand binding site.

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