Homology model of PcrV and its analysis indicated the structurally and functionally conserved regions. A. Cartoon representation of homology model of PcrV depicts a dumbbell shaped structure with N and C-terminal globular domains. Helices 7 and 12 form the grip of the dumbbell. B. ConSurf predicted the structurally and functionally conserved residues within the homology model of PcrV, in a graded manner as shown by the colour code. C. WebLogo generated sequence Logos of helix-7 and 12 of V-antigens. Sequences of helix-7 and 12 of PcrV were aligned with the sequence Logos to determine the conserved positions.