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Table 1 Kinetic parameters of interaction between deletion mutants of PcrG and PcrV, were determined by SPR

From: PcrG protects the two long helical oligomerization domains of PcrV, by an interaction mediated by the intramolecular coiled-coil region of PcrG

Kinetic parameter PcrG-PcrV ∆PcrG(1–74)-PcrV PcrG-∆PcrV(128–294) ∆PcrG(13–72)-PcrV ∆PcrG(13–72)-∆PcrV(128–294)
K A (1/M) 6.4 × 107 6.4 × 107 4.11 × 107 1.75 × 106 1.61 × 106
K D (M) 1.56 × 10-8 1.56 × 10-8 2.43 × 10-8 5.7 × 10-7 6.21 × 10-7
K a (1/MS) 4.45 × 105 3.16 × 105 5.11 × 102 1.71 × 104 2.72 × 101
K d (1/S) 6.94 × 10-3 4.91 × 10-3 1.24 × 10-5 9.78 × 10-3 1.69 × 10-5
  1. Table 1 Binding affinities, and association and dissociation rates of interaction between deletion mutants of PcrG and PcrV, were determined by SPR. Kinetic parameters of PcrG-PcrV interaction and ∆PcrG(1–74)-PcrV interaction were adapted from Nanao et al.[14].