PcrG protects the two long helical oligomerization domains of PcrV, by an interaction mediated by the intramolecular coiled-coil region of PcrG

Table 1 Kinetic parameters of interaction between deletion mutants of PcrG and PcrV, were determined by SPR

Kinetic parameter	PcrG-PcrV	∆PcrG_(1–74)-PcrV	PcrG-∆PcrV_(128–294)	∆PcrG_(13–72)-PcrV	∆PcrG_(13–72)-∆PcrV_(128–294)
K _A (1/M)	6.4 × 10⁷	6.4 × 10⁷	4.11 × 10⁷	1.75 × 10⁶	1.61 × 10⁶
K _D (M)	1.56 × 10^-8	1.56 × 10^-8	2.43 × 10^-8	5.7 × 10^-7	6.21 × 10^-7
K _a (1/MS)	4.45 × 10⁵	3.16 × 10⁵	5.11 × 10²	1.71 × 10⁴	2.72 × 10¹
K _d (1/S)	6.94 × 10^-3	4.91 × 10^-3	1.24 × 10^-5	9.78 × 10^-3	1.69 × 10^-5

Table 1 Binding affinities, and association and dissociation rates of interaction between deletion mutants of PcrG and PcrV, were determined by SPR. Kinetic parameters of PcrG-PcrV interaction and ∆PcrG_(1–74)-PcrV interaction were adapted from Nanao et al.[14].

ISSN: 1472-6807