|
Kinetic parameter
|
PcrG-PcrV
|
∆PcrG(1–74)-PcrV
|
PcrG-∆PcrV(128–294)
|
∆PcrG(13–72)-PcrV
|
∆PcrG(13–72)-∆PcrV(128–294)
|
|---|
|
K
A
(1/M)
|
6.4 × 107
|
6.4 × 107
|
4.11 × 107
|
1.75 × 106
|
1.61 × 106
|
|
K
D
(M)
|
1.56 × 10-8
|
1.56 × 10-8
|
2.43 × 10-8
|
5.7 × 10-7
|
6.21 × 10-7
|
|
K
a
(1/MS)
|
4.45 × 105
|
3.16 × 105
|
5.11 × 102
|
1.71 × 104
|
2.72 × 101
|
|
K
d
(1/S)
|
6.94 × 10-3
|
4.91 × 10-3
|
1.24 × 10-5
|
9.78 × 10-3
|
1.69 × 10-5
|
- Table 1 Binding affinities, and association and dissociation rates of interaction between deletion mutants of PcrG and PcrV, were determined by SPR. Kinetic parameters of PcrG-PcrV interaction and ∆PcrG(1–74)-PcrV interaction were adapted from Nanao et al.[14].
