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Table 3 Effect of site-directed mutagenesis on enzyme activity

From: Molecular analysis of hyperthermophilic endoglucanase Cel12B from Thermotoga maritima and the properties of its functional residues

Strain Optimum temp (°C) Specific activity (U mg-1) Relative activity (%)
Control 90 105 ± 3.4 100 ± 3.2
E64T 85 53 ± 1.3 50 ± 1.2
E64H 85 25 ± 1.0 24 ± 1.0
E64L ND 0 0
E64S 90 133 ± 2.5 127 ± 2.4
P63K ND 0 0
P83K ND 0 0
M133W ND 0 0
  1. ND: not determined. Values shown were the mean of triplicate experiments.