Table 3 Effect of site-directed mutagenesis on enzyme activity
Strain | Optimum temp (°C) | Specific activity (U mg-1) | Relative activity (%) |
|---|---|---|---|
Control | 90 | 105 ± 3.4 | 100 ± 3.2 |
E64T | 85 | 53 ± 1.3 | 50 ± 1.2 |
E64H | 85 | 25 ± 1.0 | 24 ± 1.0 |
E64L | ND | 0 | 0 |
E64S | 90 | 133 ± 2.5 | 127 ± 2.4 |
P63K | ND | 0 | 0 |
P83K | ND | 0 | 0 |
M133W | ND | 0 | 0 |