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Table 4 Identical sequences with positive antigenicity predictions

From: Screening of transgenic proteins expressed in transgenic food crops for the presence of short amino acid sequences identical to potential, IgE – binding linear epitopes of allergens

Sequence

Transgenic protein (1)

Allergen (2)

Antigenic?

(highest peak)

Part of IgE epitope?

(literature)

 

name

source

name

source

Transgenic

protein

Allergens

 
     

w = 6

w = 7

w = 6

w = 7

 

PRKGSD

Acetolactate synthase II

(mutant S4-Hra)

Tobacco Nicotiana tabacum

Amb a 1.4

Ragweed Ambrosia artemisiifolia

-

Yes

-

-

-

TSRRRR

Coat protein

Cucumber mosaic virus

ABA-1

Roundworms Ascaris lumbricoides

and A. suum

Yes

Yes

- (3)

- (3)

No (4)

EKQKEK

Coat protein

Papaya ringspot virus P

ABA-1

Roundworms Ascaris lumbricoides

and A. suum

- (5)

- (5)

Yes (6)

Yes

-

VKSEDG

Enoylpyruvate shikimate

phosphate synthase

Agrobacterium CP4

Der p 7

Housedust mite Dermatophagoides pteronyssinus

Yes

Yes

-

-

-

LAEEAD

Glyphosate oxidoreductase

Achromobacter LBAA

Pan s 1

Lobster Panulirus stimpsoni

-

-

-

Yes (7)

Yes (8)

  1. (1) Accessions: ALS: gi124369, CMV CP: gi593495, PRV CP: gi593497, CP4 EPSPS: gi8469107, GOX: gi1252836 (2) Accessions: Amb a 1.4: gi113478, gi539050, gi166445; ABA-1 (TSRRRR): gi159653, gi477301, gi2498099, gi2735096, gi2735098, gi2735100, gi2735102, gi2735104, gi2735106, gi2735108, gi2735110, gi2735112, gi2970629, gi7494507; ABA-1 (EKQKEK): gi2735108, gi2735110, gi2735112, gi2735114, gi2735116, gi2735118, gi2970629, gi7494507; Der p 7: gi1352240, gi1045602; Pan s 1: gi14285797, gi3080761 (3) Calculation not possible because TSRRRR is C-terminal sequence of the ABA-1 proteins. (4) The sequence RRRR of these allergens probably does not occur in vivo in the allergen as it would be split off from the allergen by proteases [25] (5) Sequence EKQKEK corresponds to a plateau slightly below the highest peak(s) in the antigenicity plots for the papaya ringspot virus coat protein (6) Highest score was shared by two or three peaks in the antigenicity plot for each ABA-1 protein (w = 6) (7) Highest score was shared by five peaks in the antigenicity plot for the Pan s 1 protein (window 7 amino acids) (8) Sequence LAEEAD is part of a 9-mer peptide from the shrimp tropomyosin allergen Pen i 1 that is bound by sera from shrimp allergic patients [26, 27]. This sequence has also been part of two 15-mer peptides from the shrimp allergen Pen a 1 tested for sera binding. One peptide is bound by sera from allergic individuals, whereas the other peptide is not [28].