Table 1 The secondary structure prediction in the known PKCK2 phosphorylation sites (S/T-X-X-D/E) in protein substrates. H represents prediction of helix and – represent no prediction of helix or β-sheet. Prediction by both PHD and JPRED methods have been shown in those cases with difference in the secondary structure prediction.
Protein | Secondary structure prediction at the S/T-X-X-D/E motif | |
|---|---|---|
Acetyl Co-A | Â | ---HH |
Clatharin | 11,13 | ----, ---- |
EF | 90 | --HH |
Hsp90 (beta) | 226, 255 | --HH, ---- |
Hsp90 (alpha) | 236,263 | ---H, ---H |
EIF | Â | ---- |
Ck2β |  | ---- |
DRAPP32 | 42,45,102 | ----, ----, ---- |
HPr E7 | 31,32 | ----, ---- |
Troponin T | 1 | ---- |
Glycogen synthase | 657 | ---- |
PKA RII | 74,76 | ---- |
HMG14 | 89 | ---- |
Myosin light chain | Â | --HH (PHD) -HHH(JPRED) |
ODC | 297,303 | ----, ---- |
Ppi | 87,120,121 | ---- |
Calmodulin | 81/79,101 | HHHH, --HH |
MDM2 | 267 | ---- |
P53 | 392 | ---- |
Eif 2 beta | 2,67 | ----, -HHH |
C8 subunit of MCP | 243, 250 | HHHH, ---- |
eIF5 | 387, 388 | ----,---- |
FCP1 | 457 | ---- |
Movement protein of TMV | 256,261 | ----, ---- |
CDC25B | 186,187 | ----, ---- |
FMR1 | 406 | ---- |
PTEN | 370,385 | ----, ---- |
Mengovirus leader protein | 47 | ---- |
β arrestin 2 | 383 | --HH (JPRED) ----(PHD) |
Cyclin H | 315 | ---- |
UBC3B | 233 | ---- |
Topoisomerase II α | 1342 | ---- |
Rotavirus NSP5 | 153,155,163,165 | ----, ----, ----, ---- |
TF III A | 16 | ---- |
HIV1 Rev | 5,8 | ---- (JPRED) HHHH (PHD), -HHH (JPRED) HHHH(PHD) |
HDAC1 | 421,423 | ----(JPRED) ---H (PHD), ----(JPRED) HHHH (PHD) |
CDC34 | 203,222,231,233,236 | ----, ----, ----, ----, ---- |
eIF epsilon | 712,713 | ----, ---- |
FAF1 | 289,291 | ----, ---- |
VIPR-RP | 69,71,110 | ----, ----(JPRED) --HH(PHD), ---- |
NDPKA | 122 | --HH |