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Figure 2 | BMC Structural Biology

Figure 2

From: Functional asymmetry in the lysyl-tRNA synthetase explored by molecular dynamics, free energy calculations and experiment

Figure 2

Fluorimetric titration of LysU (a) Fluorimetric titration of LysU with lysine observing change in fluorescence under Michaelis-Menten conditions. The enzyme is at a concentration (0.5 μM) significantly below the dissociation constant. The continuous line is a simple Michaelis-Menten single site fit to the dataset. (b) Fluorimetric titration of LysU with lysine under stoichiometric conditions. The enzyme is at a concentration (17 μM) significantly above the value of the dissociation constant. The two straight lines represent fits on the linear regions of their data, the intercept results in the position of the Klotz transition represented by the dotted line.

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