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Figure 3 | BMC Structural Biology

Figure 3

From: Functional asymmetry in the lysyl-tRNA synthetase explored by molecular dynamics, free energy calculations and experiment

Figure 3

ITC data of ligand binding to LysU (a) The lower trace shows a calorimetric titration profile of aliquots of lysine being injected into 6 μM LysU (concentration of monomer) in the presence of 10 mM MgCl2 in 25 mM TRIS-HCl pH 8.0 at 20°C. The upper trace is the control of 225 μM lysine being injected into the same buffer under identical conditions in the absence of LysU. (b) A least squares fit of the data, the heat absorbed per mole of titrant versus the ratio of concentration of lysine to the monomeric concentration of LysU. (c) The lower trace is a calorimetric titration profile of aliquots of 250 μM AMPPCP being injected into 20 μM LysU (concentration of monomer) in the presence of 10 mM MgCl2 and 1 mM lysine in 25 mM TRIS-HCl pH 8.0 at 20°C. The upper trace is the same titration in the absence of lysine. (d) A least squares fit of the data, the heat absorbed per mole of titrant versus the ratio of concentration of AMPPCP to the monomeric concentration of LysU.

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