Functional asymmetry in the lysyl-tRNA synthetase explored by molecular dynamics, free energy calculations and experiment

Table 1 Experimentally determined thermodynamic parameters of binding events.

Binding Event	Method	Stoichiometry to monomer	K_D (μM)	ΔH (cal/mol)	ΔS (cal/mol·K)
Lysine	Fluorescence	0.98 ± 0.10	7.96 ± 1.40	-	-
Lysine	ITC	0.87 ± 0.14	4.95 ± 2.54	-1.07 × 10⁴± 2104	-12.4
AMPPCP	ITC	0.51 ± 0.01	1.22 ± 0.15	- 5602 ± 168	+ 7.94

Lysine is binding to LysU in presence of 10 mM MgCl₂, AMPPCP is binding to LysU in presence of 10 mM MgCl₂ and 1 mM lysine.

ISSN: 1472-6807