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Table 1 Data Collection and Refinement.

From: Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II

Crystal

VPS25-SEMET

 

VPS25-NATIVE

 

Space Group

P212121

 

P212121

 

Wavelength

0.97914

 

0.931

 

Unit Cell (Å)

a

53.44

 

53.36

 

b

124.11

 

123.66

 

c

139.48

 

140.30

 

Resolution (outer shell) (Å)

100-3.20 (3.31-3.20)

 

100 - 3.10 (3.21-3.10)

 

Total Reflections (outer shell)

111407 (10016)

 

65477 (6064)

 

Unique Reflections

29230

 

16330

 

Completeness (%) (outer shell)

98.9 (93.1)

 

93.1 (91.3)

 

Rmerge (outer shell)

0.090 (0.335)

 

0.053 (0.307)

 

Average I/sigma (outer shell)

12.3 (4.9)

 

20.9 (4.9)

 

Phasing

Number of Se Sites

14

   

SOLVE FOM

0.351

   

RESOLVE FOM (ncs)

0.694

   

Refinement

Resolution (outer shell) (Å)

25.0-3.10 (3.29-3.10)

   

Number of reflections (test set)

16404 (790)

   

R factor

0.275

   

Free R factor

0.327

   

Number of protein/solvent atoms

5760/16

   

Average B factor (Å2)

51.3

   

Rms deviation bond lengths (Å)

0.009

   

Ramachandron

Mol A

Mol B

MolC

MolD

Most favored

Additionally favored

83.8

15.7

79.5

20.5

82.1

17.9

67.2

32.8