From: Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II
Crystal | VPS25-SEMET | VPS25-NATIVE | ||
---|---|---|---|---|
Space Group | P212121 | P212121 | ||
Wavelength | 0.97914 | 0.931 | ||
Unit Cell (Å) | ||||
a | 53.44 | 53.36 | ||
b | 124.11 | 123.66 | ||
c | 139.48 | 140.30 | ||
Resolution (outer shell) (Å) | 100-3.20 (3.31-3.20) | 100 - 3.10 (3.21-3.10) | ||
Total Reflections (outer shell) | 111407 (10016) | 65477 (6064) | ||
Unique Reflections | 29230 | 16330 | ||
Completeness (%) (outer shell) | 98.9 (93.1) | 93.1 (91.3) | ||
Rmerge (outer shell) | 0.090 (0.335) | 0.053 (0.307) | ||
Average I/sigma (outer shell) | 12.3 (4.9) | 20.9 (4.9) | ||
Phasing | ||||
Number of Se Sites | 14 | |||
SOLVE FOM | 0.351 | |||
RESOLVE FOM (ncs) | 0.694 | |||
Refinement | ||||
Resolution (outer shell) (Å) | 25.0-3.10 (3.29-3.10) | |||
Number of reflections (test set) | 16404 (790) | |||
R factor | 0.275 | |||
Free R factor | 0.327 | |||
Number of protein/solvent atoms | 5760/16 | |||
Average B factor (Å2) | 51.3 | |||
Rms deviation bond lengths (Å) | 0.009 | |||
Ramachandron | Mol A | Mol B | MolC | MolD |
Most favored Additionally favored | 83.8 15.7 | 79.5 20.5 | 82.1 17.9 | 67.2 32.8 |