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Table 1 Data Collection and Refinement.

From: Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II

Crystal VPS25-SEMET   VPS25-NATIVE  
Space Group P212121   P212121  
Wavelength 0.97914   0.931  
Unit Cell (Å)
a 53.44   53.36  
b 124.11   123.66  
c 139.48   140.30  
Resolution (outer shell) (Å) 100-3.20 (3.31-3.20)   100 - 3.10 (3.21-3.10)  
Total Reflections (outer shell) 111407 (10016)   65477 (6064)  
Unique Reflections 29230   16330  
Completeness (%) (outer shell) 98.9 (93.1)   93.1 (91.3)  
Rmerge (outer shell) 0.090 (0.335)   0.053 (0.307)  
Average I/sigma (outer shell) 12.3 (4.9)   20.9 (4.9)  
Phasing
Number of Se Sites 14    
SOLVE FOM 0.351    
RESOLVE FOM (ncs) 0.694    
Refinement
Resolution (outer shell) (Å) 25.0-3.10 (3.29-3.10)    
Number of reflections (test set) 16404 (790)    
R factor 0.275    
Free R factor 0.327    
Number of protein/solvent atoms 5760/16    
Average B factor (Å2) 51.3    
Rms deviation bond lengths (Å) 0.009    
Ramachandron Mol A Mol B MolC MolD
Most favored Additionally favored 83.8 15.7 79.5 20.5 82.1 17.9 67.2 32.8