Figure 4

RT-PCR results for 5'-termini of trypsin and collagenase. A. Agarose gel electrophoresis of PCR products generated using 5'-RACE primer for trypsin and step-out primer system. B. Agarose gel electrophoresis of PCR products generated using 5'-RACE primer for collagenase and step-out primer system. About 1 ng of the first strand cDNA was used as a starting material for PCR reaction. Cycling was performed in a MJ Research PTC-200 Thermocycler in calculated mode: 25 cycles for trypsin and 26 cycles for collagenase were made using cycling profile: 95°C for 8 s, 65°C for 10 s, and 72°C for 2 min.