Figure 1

High-throughput domain characterisation and evaluation by 2D ¹H-¹⁵N-HSQC spectroscopy. Analytical results for the SEP-domain (residues 171 – 270 from protein AAH02801.1), the N-terminal DCX domain from protein AAC31696.1 (residues 90 – 186) and the L27-domain from protein AAG34117.1 (residues 13 – 68) are shown. Samples for SDS-PAGE, analytical HIC and 1D ¹H-NMR spectra were obtained from two separate automated small-scale purifications of 1 ml cultures. 2D ¹H-¹⁵N-HSQC spectra resulted from the purification of a 400 ml culture. SDS-PAGE lanes: M: marker; I: NiNTA-bound protein eluted with imidazole for analytical HIC; E: NiNTA-bound protein eluted with EDTA for 1D ¹H-NMR analysis.