Figure 3
Sedimentation equilibrium analysis of the C-domain. (A). Sedimentation equilibrium of recombinant C-domain at 50000 rpm. Equilibrium A280-radius profiles for three different cell compartments, each one containing the protein at different concentrations. The lines are the best (global) fit to a single species model. The residuals of the fit are shown in the three upper panels.(B). Sedimentation equilibrium analysis of the C-domain in the presence of 1 mM SDS. The sample was run at 45000 rpm. The line represents the best fit to a two state equilibrium model. The residuals of the fit are shown in the upper panel.